CPLM-019276

CPLM 1.0 - Compendium of Protein Lysine Modification

Tag

Content

CPLM ID

CPLM-019276

UniProt Accession

TRM2_HUMAN; Q96GJ1; A6NDG5; A6NEI9; A6NMG6; Q5JPF0; Q5JVY6; Q96HU7; Q96IH9; Q9H9K2

Genbank Protein ID

AK022749; AL832849; AL133275; AL109952; Z97985; AL133275; AL109952; Z97985; Z97985; AL109952; AL133275; Z97985; AL109952; AL133275; AL109952; AL133275; Z97985; AL109952; AL133275; Z97985; CH471115; CH471115; BC007526; BC008067; BC009437; BC020116; BC034272

Genbank Nucleotide ID

BAB14223.1; CAI46112.1; CAI42155.1; CAI42155.1; CAI42155.1; CAI42156.1; CAI42156.1; CAI42156.1; CAI42658.1; CAI42658.1; CAI42658.1; CAI42657.1; CAI42657.1; CAI42657.1; CAI42997.1; CAI42997.1; CAI42997.1; CAI42998.1; CAI42998.1; CAI42998.1; EAX02831.1; EAX02837.1; AAH07526.1; AAH08067.2; AAH09437.1; AAH20116.1; AAH34272.1

Protein Name

tRNA (uracil(54)-C(5))-methyltransferase homolog

Protein Synonyms/Alias

TRM2 homolog

Gene Name

TRMT2B

Gene Synonyms/Alias

CXorf34

Created Date

July 27, 2013

Organism

Homo sapiens (Human)

NCBI Taxa ID

9606

Lysine Modification

Position	Peptide	Type	References
263	HVQKEIVKEFFIRGP	ubiquitination	[1]
403	FHTGQAEKILPGLLK	ubiquitination	[1]
410	KILPGLLKSKEDGQS	ubiquitination	[1]
412	LPGLLKSKEDGQSIV	ubiquitination	[1]

Reference

[1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]

Functional Description

Probable S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the formation of 5-methyl-uridine at position 54 (m5U54) in all tRNA. May also have a role in tRNA stabilization or maturation (By similarity).

Sequence Annotation

ACT_SITE 451 451 Nucleophile (By similarity).
BINDING 323 323 S-adenosyl-L-methionine (By similarity).
BINDING 373 373 S-adenosyl-L-methionine (By similarity).

Keyword

Alternative splicing; Complete proteome; Methyltransferase; Polymorphism; Reference proteome; S-adenosyl-L-methionine; Transferase; tRNA processing.

Sequence Source

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

Protein Length

504 AA

Protein Sequence

MAGLKRRVPL HSLRYFISMV GLFSKPGLLP WYARNPPGWS QLFLGTVCKG DFTRVIATKC 60
QKGQKSQKKP SHLGPLDGSW QERLADVVTP LWRLSYEEQL KVKFAAQKKI LQRLESYIQM 120
LNGVSVTTAV PKSERLSCLL HPIIPSPVIN GYRNKSTFSV NRGPDGNPKT VGFYLGTWRD 180
GNVVCVQSNH LKNIPEKHSQ VAQYYEVFLR QSPLEPCLVF HEGGYWRELT VRTNSQGHTM 240
AIITFHPQKL SQEELHVQKE IVKEFFIRGP GAACGLTSLY FQESTMTRCS HQQSPYQLLF 300
GEPYIFEELL SLKIRISPDA FFQINTAGAE MLYRTVGELT GVNSDTILLD ICCGTGVIGL 360
SLAQHTSRVL GIELLEQAVE DARWTAAFNG ITNSEFHTGQ AEKILPGLLK SKEDGQSIVA 420
VVNPARAGLH YKVIQAIRNF RAIHTLVFVS CKLHGESTRN VIELCCPPDP AKKLLGEPFV 480
LQQAVPVDLF PHTPHCELVL LFTR 504

Gene Ontology

GO:0005739; C:mitochondrion; IEA:Compara.
GO:0030697; F:S-adenosylmethionine-dependent tRNA (m5U54) methyltransferase activity; IEA:EC.

Interpro

IPR025714; Methyltranfer_dom.
IPR025823; tRNA_(uracil-5-)_MeTrfase_met.

Pfam

PF13847; Methyltransf_31

SMART

PROSITE

PRINTS