UniProt Accession

 SGF29_YEAST; P25554; D6VR02; P87008 

Genbank Protein ID

 X59720; BK006937 

Genbank Nucleotide ID

 CAA42349.1; DAA07471.1 

Protein Name

 SAGA-associated factor 29 

Protein Synonyms/Alias

 29 kDa SAGA-associated factor; SAGA histone acetyltransferase complex 29 kDa subunit 

Gene Name

 SGF29 

Gene Synonyms/Alias

 YCL010C; YCL10C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
174	DELGNSGKVYKCNRK	acetylation	[1]
193	IPPGFPTKNYPPGTK	acetylation	[1]
200	KNYPPGTKVLARYPE	acetylation	[1]
220	PAIVIGTKRDGTCRL	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Functions as component of the transcription regulatory histone acetylation (HAT) complexes SAGA and SLIK. SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3 and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). SLIK is proposed to have partly overlapping functions with SAGA. It preferentially acetylates methylated histone H3, at least after activation at the GAL1-10 locus. 

Sequence Annotation

 DOMAIN 121 255 SGF29 C-terminal.
 REGION 163 165 Histone H3K4me3 N-terminus binding.
 REGION 207 210 Histone H3K4me3 N-terminus binding.
 REGION 229 232 Histone H3K4me3 binding.
 BINDING 205 205 Histone H3K4me3.
 BINDING 212 212 Histone H3K4me3.
 MOD_RES 139 139 Phosphoserine.  

Keyword

 3D-structure; Chromatin regulator; Complete proteome; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 259 AA 

Protein Sequence

Gene Ontology

 GO:0005671; C:Ada2/Gcn5/Ada3 transcription activator complex; IMP:SGD.
 GO:0000124; C:SAGA complex; IDA:SGD.
 GO:0035064; F:methylated histone residue binding; IDA:SGD.
 GO:0001135; F:RNA polymerase II transcription factor recruiting transcription factor activity; IMP:SGD.
 GO:0034629; P:cellular protein complex localization; IMP:SGD.
 GO:0044154; P:histone H3-K14 acetylation; IMP:SGD.
 GO:0043971; P:histone H3-K18 acetylation; IMP:SGD.
 GO:0043970; P:histone H3-K9 acetylation; IMP:SGD.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IMP:SGD. 

Interpro

 IPR010750; SGF29_tudor-like_dom. 

Pfam

 PF07039; DUF1325 

SMART

  

PROSITE

 PS51518; SGF29_C 

PRINTS