CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018785
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein FAN 
Protein Synonyms/Alias
 Factor associated with neutral sphingomyelinase activation; Factor associated with N-SMase activation 
Gene Name
 NSMAF 
Gene Synonyms/Alias
 FAN 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
76SISQPIIKIPLRDCIubiquitination[1]
84IPLRDCIKIGKHGENubiquitination[1]
368KPVGALNKERLERLLubiquitination[1, 2]
750PAEMPGTKRHHFDLLubiquitination[3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Couples the p55 TNF-receptor (TNF-R55 / TNFR1) to neutral sphingomyelinase (N-SMASE). Specifically binds to the N- smase activation domain of TNF-R55. May regulate ceramide production by N-SMASE. 
Sequence Annotation
 DOMAIN 176 247 GRAM.
 DOMAIN 290 575 BEACH.
 REPEAT 628 658 WD 1.
 REPEAT 670 700 WD 2.
 REPEAT 712 740 WD 3.
 REPEAT 761 791 WD 4.
 REPEAT 803 833 WD 5.
 REPEAT 884 914 WD 6.  
Keyword
 Alternative splicing; Complete proteome; Polymorphism; Reference proteome; Repeat; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 917 AA 
Protein Sequence
MAFIRKKQQE QQLQLYSKER FSLLLLNLEE YYFEQHRANH ILHKGSHHER KIRGSLKICS 60
KSVIFEPDSI SQPIIKIPLR DCIKIGKHGE NGANRHFTKA KSGGISLIFS QVYFIKEHNV 120
VAPYKIERGK MEYVFELDVP GKVEDVVETL LQLHRASCLD KLGDQTAMIT AILQSRLART 180
SFDKNRFQNI SEKLHMECKA EMVTPLVTNP GHVCITDTNL YFQPLNGYPK PVVQITLQDV 240
RRIYKRRHGL MPLGLEVFCT EDDLCSDIYL KFYEPQDRDD LYFYIATYLE HHVAEHTAES 300
YMLQWQRGHL SNYQYLLHLN NLADRSCNDL SQYPVFPWII HDYSSSELDL SNPGTFRDLS 360
KPVGALNKER LERLLTRYQE MPEPKFMYGS HYSSPGYVLF YLVRIAPEYM LCLQNGRFDN 420
ADRMFNSIAE TWKNCLDGAT DFKELIPEFY GDDVSFLVNS LKLDLGKRQG GQMVDDVELP 480
PWASSPEDFL QKSKDALESN YVSEHLHEWI DLIFGYKQKG SDAVGAHNVF HPLTYEGGVD 540
LNSIQDPDEK VAMLTQILEF GQTPKQLFVT PHPRRITPKF KSLSQTSSYN ASMADSPGEE 600
SFEDLTEESK TLAWNNITKL QLHEHYKIHK EAVTGITVSR NGSSVFTTSQ DSTLKMFSKE 660
SKMLQRSISF SNMALSSCLL LPGDATVITS SWDNNVYFYS IAFGRRQDTL MGHDDAVSKI 720
CWHDNRLYSA SWDSTVKVWS GVPAEMPGTK RHHFDLLAEL EHDVSVDTIS LNAASTLLVS 780
GTKEGTVNIW DLTTATLMHQ IPCHSGIVCD TAFSPDSRHV LSTGTDGCLN VIDVQTGMLI 840
SSMTSDEPQR CFVWDGNSVL SGSQSGELLV WDLLGAKISE RIQGHTGAVT CIWMNEQCSS 900
IITGGEDRQI IFWKLQY 917 
Gene Ontology
 GO:0005737; C:cytoplasm; TAS:ProtInc.
 GO:0005057; F:receptor signaling protein activity; TAS:ProtInc.
 GO:0006672; P:ceramide metabolic process; TAS:ProtInc. 
Interpro
 IPR000409; BEACH_dom.
 IPR004182; GRAM.
 IPR023362; PH-BEACH_dom.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF02138; Beach
 PF02893; GRAM
 PF00400; WD40 
SMART
 SM01026; Beach
 SM00568; GRAM
 SM00320; WD40 
PROSITE
 PS50197; BEACH
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS