CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015638
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 La-related protein 4 
Protein Synonyms/Alias
 La ribonucleoprotein domain family member 4 
Gene Name
 LARP4 
Gene Synonyms/Alias
 PP13296 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
216TTPIEEVKGLFKSENubiquitination[1, 2]
220EEVKGLFKSENCPKVubiquitination[1, 2]
265EVKTFQGKPIMARIKubiquitination[1]
272KPIMARIKAINTFFAubiquitination[1]
280AINTFFAKNGYRLMDubiquitination[1, 3]
372NMGRPFQKNRVKPQFubiquitination[1, 2, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 Plays a role in the regulation of cell morphology and cytoskeletal organization. 
Sequence Annotation
 DOMAIN 113 202 HTH La-type RNA-binding.
 DOMAIN 203 281 RRM.
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 392 392 Phosphoserine.
 MOD_RES 583 583 Phosphoserine.
 MOD_RES 647 647 Phosphoserine.
 MOD_RES 649 649 Phosphothreonine.
 MOD_RES 722 722 Phosphoserine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Complete proteome; Direct protein sequencing; Phosphoprotein; Polymorphism; Reference proteome; RNA-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 724 AA 
Protein Sequence
MLLFVEQVAS KGTGLNPNAK VWQEIAPGNT DATPVTHGTE SSWHEIAATS GAHPEGNAEL 60
SEDICKEYEV MYSSSCETTR NTTGIEESTD GMILGPEDLS YQIYDVSGES NSAVSTEDLK 120
ECLKKQLEFC FSRENLSKDL YLISQMDSDQ FIPIWTVANM EEIKKLTTDP DLILEVLRSS 180
PMVQVDEKGE KVRPSHKRCI VILREIPETT PIEEVKGLFK SENCPKVISC EFAHNSNWYI 240
TFQSDTDAQQ AFKYLREEVK TFQGKPIMAR IKAINTFFAK NGYRLMDSSI YSHPIQTQAQ 300
YASPVFMQPV YNPHQQYSVY SIVPQSWSPN PTPYFETPLA PFPNGSFVNG FNSPGSYKTN 360
AAAMNMGRPF QKNRVKPQFR SSGGSEHSTE GSVSLGDGQL NRYSSRNFPA ERHNPTVTGH 420
QEQTYLQKET STLQVEQNGD YGRGRRTLFR GRRRREDDRI SRPHPSTAES KAPTPKFDLL 480
ASNFPPLPGS SSRMPGELVL ENRMSDVVKG VYKEKDNEEL TISCPVPADE QTECTSAQQL 540
NMSTSSPCAA ELTALSTTQQ EKDLIEDSSV QKDGLNQTTI PVSPPSTTKP SRASTASPCN 600
NNINAATAVA LQEPRKLSYA EVCQKPPKEP SSVLVQPLRE LRSNVVSPTK NEDNGAPENS 660
VEKPHEKPEA RASKDYSGFR GNIIPRGAAG KIREQRRQFS HRAIPQGVTR RNGKEQYVPP 720
RSPK 724 
Gene Ontology
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0007010; P:cytoskeleton organization; IMP:UniProtKB.
 GO:0022604; P:regulation of cell morphogenesis; IMP:UniProtKB. 
Interpro
 IPR006630; Lupus_La_RNA-bd.
 IPR000504; RRM_dom.
 IPR011991; WHTH_DNA-bd_dom. 
Pfam
 PF05383; La 
SMART
 SM00715; LA
 SM00360; RRM 
PROSITE
 PS50961; HTH_LA
 PS50102; RRM 
PRINTS