CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005837
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein disulfide-isomerase A3 
Protein Synonyms/Alias
 58 kDa glucose-regulated protein; 58 kDa microsomal protein; p58; Disulfide isomerase ER-60; Endoplasmic reticulum resident protein 57; ER protein 57; ERp57; Endoplasmic reticulum resident protein 60; ER protein 60; ERp60 
Gene Name
 PDIA3 
Gene Synonyms/Alias
 ERP57; ERP60; GRP58 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
82KGIVPLAKVDCTANTubiquitination[1]
94ANTNTCNKYGVSGYPubiquitination[1, 2]
104VSGYPTLKIFRDGEEubiquitination[1, 2]
226TVAYTEQKMTSGKIKacetylation[3]
296KFLDAGHKLNFAVASubiquitination[1, 2]
305NFAVASRKTFSHELSubiquitination[1, 2]
335IRTAKGEKFVMQEEFubiquitination[1, 2]
362DYFDGNLKRYLKSEPubiquitination[1, 2]
425ELGEKLSKDPNIVIAubiquitination[2]
Reference
 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786] 
Functional Description
  
Sequence Annotation
 DOMAIN 25 133 Thioredoxin 1.
 DOMAIN 343 485 Thioredoxin 2.
 MOTIF 502 505 Prevents secretion from ER (By
 ACT_SITE 57 57 Nucleophile (By similarity).
 ACT_SITE 60 60 Nucleophile (By similarity).
 ACT_SITE 406 406 Nucleophile (By similarity).
 ACT_SITE 409 409 Nucleophile (By similarity).
 DISULFID 57 60 Redox-active; alternate (By similarity).
 DISULFID 57 57 Interchain (with C-115 in TAPBP);
 DISULFID 85 92
 DISULFID 406 409 Redox-active (By similarity).  
Keyword
 3D-structure; Complete proteome; Direct protein sequencing; Disulfide bond; Endoplasmic reticulum; Isomerase; Polymorphism; Redox-active center; Reference proteome; Repeat; Signal. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 505 AA 
Protein Sequence
MRLRRLALFP GVALLLAAAR LAAASDVLEL TDDNFESRIS DTGSAGLMLV EFFAPWCGHC 60
KRLAPEYEAA ATRLKGIVPL AKVDCTANTN TCNKYGVSGY PTLKIFRDGE EAGAYDGPRT 120
ADGIVSHLKK QAGPASVPLR TEEEFKKFIS DKDASIVGFF DDSFSEAHSE FLKAASNLRD 180
NYRFAHTNVE SLVNEYDDNG EGIILFRPSH LTNKFEDKTV AYTEQKMTSG KIKKFIQENI 240
FGICPHMTED NKDLIQGKDL LIAYYDVDYE KNAKGSNYWR NRVMMVAKKF LDAGHKLNFA 300
VASRKTFSHE LSDFGLESTA GEIPVVAIRT AKGEKFVMQE EFSRDGKALE RFLQDYFDGN 360
LKRYLKSEPI PESNDGPVKV VVAENFDEIV NNENKDVLIE FYAPWCGHCK NLEPKYKELG 420
EKLSKDPNIV IAKMDATAND VPSPYEVRGF PTIYFSPANK KLNPKKYEGG RELSDFISYL 480
QREATNPPVI QEEKPKKKKK AQEDL 505 
Gene Ontology
 GO:0009986; C:cell surface; IDA:MGI.
 GO:0005788; C:endoplasmic reticulum lumen; TAS:Reactome.
 GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
 GO:0004197; F:cysteine-type endopeptidase activity; TAS:ProtInc.
 GO:0009055; F:electron carrier activity; IEA:InterPro.
 GO:0004629; F:phospholipase C activity; TAS:ProtInc.
 GO:0003756; F:protein disulfide isomerase activity; TAS:ProtInc.
 GO:0015035; F:protein disulfide oxidoreductase activity; IEA:InterPro.
 GO:0002479; P:antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; TAS:Reactome.
 GO:0045454; P:cell redox homeostasis; IEA:InterPro.
 GO:0006662; P:glycerol ether metabolic process; IEA:InterPro.
 GO:0043065; P:positive regulation of apoptotic process; IEA:Compara.
 GO:0043687; P:post-translational protein modification; TAS:Reactome.
 GO:0006606; P:protein import into nucleus; TAS:ProtInc.
 GO:0018279; P:protein N-linked glycosylation via asparagine; TAS:Reactome.
 GO:0006621; P:protein retention in ER lumen; TAS:ProtInc.
 GO:0007165; P:signal transduction; TAS:ProtInc. 
Interpro
 IPR005788; Disulphide_isomerase.
 IPR005792; Prot_disulphide_isomerase.
 IPR005746; Thioredoxin.
 IPR012336; Thioredoxin-like_fold.
 IPR017937; Thioredoxin_CS.
 IPR013766; Thioredoxin_domain. 
Pfam
 PF00085; Thioredoxin 
SMART
  
PROSITE
 PS00194; THIOREDOXIN_1
 PS51352; THIOREDOXIN_2 
PRINTS
 PR00421; THIOREDOXIN.