UniProt Accession

 GSH0_MOUSE; O09172 

Genbank Protein ID

 U95053; AF149060; AF149054; AF149055; AF149056; AF149057; AF149058; AF149059; AK028236 

Genbank Nucleotide ID

 AAB96893.1; AAG15424.1; AAG15424.1; AAG15424.1; AAG15424.1; AAG15424.1; AAG15424.1; AAG15424.1; BAC25831.1 

Protein Name

 Glutamate--cysteine ligase regulatory subunit 

Protein Synonyms/Alias

 GCS light chain; Gamma-ECS regulatory subunit; Gamma-glutamylcysteine synthetase regulatory subunit; Glutamate--cysteine ligase modifier subunit 

Gene Name

 Gclm 

Gene Synonyms/Alias

 Glclr 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
90	PDEREEMKVSAKLFI	acetylation	[1]
90	PDEREEMKVSAKLFI	ubiquitination	[2]
263	SRGIIKSKGYILQAK	acetylation	[3]
263	SRGIIKSKGYILQAK	phosphoglycerylation	[4]
263	SRGIIKSKGYILQAK	ubiquitination	[2]
270	KGYILQAKRRGS***	ubiquitination	[2]

Reference

 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [4] Functional lysine modification by an intrinsically reactive primary glycolytic metabolite.
 Moellering RE, Cravatt BF.
 Science. 2013 Aug 2;341(6145):549-53. [PMID: 23908237] 

Functional Description

  

Sequence Annotation

 MOD_RES 263 263 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Complete proteome; Glutathione biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 274 AA 

Protein Sequence

Gene Ontology

 GO:0017109; C:glutamate-cysteine ligase complex; IDA:MGI.
 GO:0004357; F:glutamate-cysteine ligase activity; IEA:Compara.
 GO:0035226; F:glutamate-cysteine ligase catalytic subunit binding; ISS:UniProtKB.
 GO:0006534; P:cysteine metabolic process; IMP:MGI.
 GO:0006536; P:glutamate metabolic process; ISS:UniProtKB.
 GO:0006750; P:glutathione biosynthetic process; ISS:UniProtKB.
 GO:0043066; P:negative regulation of apoptotic process; IGI:MGI.
 GO:0043524; P:negative regulation of neuron apoptotic process; IEA:Compara.
 GO:0035229; P:positive regulation of glutamate-cysteine ligase activity; IDA:MGI.
 GO:0050880; P:regulation of blood vessel size; ISS:UniProtKB.
 GO:0051900; P:regulation of mitochondrial depolarization; IGI:MGI.
 GO:0042493; P:response to drug; ISS:UniProtKB.
 GO:0051409; P:response to nitrosative stress; IEA:Compara.
 GO:0006979; P:response to oxidative stress; ISS:UniProtKB. 

Interpro

 IPR023210; NADP_OxRdtase_dom. 

Pfam

 PF00248; Aldo_ket_red 

SMART

  

PROSITE

  

PRINTS