UniProt Accession

 ULA1_HUMAN; Q13564; A6NFN4; B2R700 

Genbank Protein ID

 U50939; AY197612; AK298159; AK312784; AC004638; AC044802; AL136798; CH471092; BC000480; BC013301 

Genbank Nucleotide ID

 AAC50477.1; AAP35030.1; BAH12735.1; BAG35647.1; AAC23784.1; CAB66732.1; EAW83042.1; AAH00480.1; AAH13301.1 

Protein Name

 NEDD8-activating enzyme E1 regulatory subunit 

Protein Synonyms/Alias

 Amyloid beta precursor protein-binding protein 1, 59 kDa; APP-BP1; Amyloid protein-binding protein 1; Proto-oncogene protein 1 

Gene Name

 NAE1 

Gene Synonyms/Alias

 APPBP1; HPP1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
6	**MAQLGKLLKEQKY	acetylation	[1]
12	GKLLKEQKYDRQLRL	ubiquitination	[2]
167	GYMRIIIKEHPVIES	ubiquitination	[2, 3]
187	LEDLRLDKPFPELRE	ubiquitination	[3]
253	LIRQGILKNENGAPE	ubiquitination	[2, 4]
299	DRCINITKQTPSFWI	ubiquitination	[3]
312	WILARALKEFVAKEG	ubiquitination	[2, 3]
317	ALKEFVAKEGQGNLP	ubiquitination	[2, 3, 4]
338	DMIADSGKYIKLQNV	ubiquitination	[2]
341	ADSGKYIKLQNVYRE	ubiquitination	[3]
363	AVGNHVAKLLQSIGQ	ubiquitination	[2, 3]
378	APESISEKELKLLCS	ubiquitination	[2, 4]
381	SISEKELKLLCSNSA	ubiquitination	[3]
456	QVEEDIGKLKSCLTG	ubiquitination	[2]

Reference

 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 

Functional Description

 Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation. 

Sequence Annotation

 REGION 331 344 Interaction with UBA3.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 6 6 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Alternative splicing; Apoptosis; Cell cycle; Cell membrane; Complete proteome; Membrane; Polymorphism; Reference proteome; Ubl conjugation; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 534 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; TAS:ProtInc.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0003824; F:catalytic activity; IEA:InterPro.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0006260; P:DNA replication; IDA:UniProtKB.
 GO:0033314; P:mitotic DNA replication checkpoint; IDA:UniProtKB.
 GO:0045116; P:protein neddylation; IDA:UniProtKB.
 GO:0043523; P:regulation of neuron apoptotic process; IDA:UniProtKB.
 GO:0007165; P:signal transduction; TAS:ProtInc. 

Interpro

 IPR009036; Molybdenum_cofac_synth_MoeB.
 IPR016040; NAD(P)-bd_dom.
 IPR000594; ThiF_NAD_FAD-bd. 

Pfam

 PF00899; ThiF 

SMART

  

PROSITE

  

PRINTS