CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009068
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Beta-secretase 1 
Protein Synonyms/Alias
 Aspartyl protease 2; ASP2; Asp 2; Beta-site amyloid precursor protein cleaving enzyme 1; Beta-site APP cleaving enzyme 1; Memapsin-2; Membrane-associated aspartic protease 2 
Gene Name
 Bace1 
Gene Synonyms/Alias
 Bace 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
126STYRDLRKGVYVPYTacetylation[1]
275EINGQDLKMDCKEYNacetylation[1]
279QDLKMDCKEYNYDKSacetylation[1]
285CKEYNYDKSIVDSGTacetylation[1]
299TTNLRLPKKVFEAAVacetylation[1]
300TNLRLPKKVFEAAVKacetylation[1]
307KVFEAAVKSIKAASSacetylation[1]
Reference
 [1] A reversible form of lysine acetylation in the ER and Golgi lumen controls the molecular stabilization of BACE1.
 Costantini C, Ko MH, Jonas MC, Puglielli L.
 Biochem J. 2007 Nov 1;407(3):383-95. [PMID: 17425515] 
Functional Description
 Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase (By similarity). 
Sequence Annotation
 REGION 479 501 Interaction with RTN3 (By similarity).
 ACT_SITE 93 93 By similarity.
 ACT_SITE 289 289 By similarity.
 CARBOHYD 153 153 N-linked (GlcNAc...) (Potential).
 CARBOHYD 172 172 N-linked (GlcNAc...) (Potential).
 CARBOHYD 223 223 N-linked (GlcNAc...) (Potential).
 CARBOHYD 354 354 N-linked (GlcNAc...) (Potential).
 DISULFID 216 420 By similarity.
 DISULFID 278 443 By similarity.
 DISULFID 330 380 By similarity.  
Keyword
 Aspartyl protease; Complete proteome; Cytoplasmic vesicle; Direct protein sequencing; Disulfide bond; Endoplasmic reticulum; Endosome; Glycoprotein; Golgi apparatus; Hydrolase; Membrane; Protease; Reference proteome; Signal; Transmembrane; Transmembrane helix; Zymogen. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 501 AA 
Protein Sequence
MAPALHWLLL WVGSGMLPAQ GTHLGIRLPL RSGLAGPPLG LRLPRETDEE SEEPGRRGSF 60
VEMVDNLRGK SGQGYYVEMT VGSPPQTLNI LVDTGSSNFA VGAAPHPFLH RYYQRQLSST 120
YRDLRKGVYV PYTQGKWEGE LGTDLVSIPH GPNVTVRANI AAITESDKFF INGSNWEGIL 180
GLAYAEIARP DDSLEPFFDS LVKQTHIPNI FSLQLCGAGF PLNQTEALAS VGGSMIIGGI 240
DHSLYTGSLW YTPIRREWYY EVIIVRVEIN GQDLKMDCKE YNYDKSIVDS GTTNLRLPKK 300
VFEAAVKSIK AASSTEKFPD GFWLGEQLVC WQAGTTPWNI FPVISLYLMG EVTNQSFRIT 360
ILPQQYLRPV EDVATSQDDC YKFAVSQSST GTVMGAVIME GFYVVFDRAR KRIGFAVSAC 420
HVHDEFRTAA VEGPFVTADM EDCGYNIPQT DESTLMTIAY VMAAICALFM LPLCLMVCQW 480
RCLRCLRHQH DDFADDISLL K 501 
Gene Ontology
 GO:0030424; C:axon; IDA:MGI.
 GO:0009986; C:cell surface; IEA:UniProtKB-SubCell.
 GO:0031410; C:cytoplasmic vesicle; IDA:MGI.
 GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
 GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
 GO:0005768; C:endosome; ISS:UniProtKB.
 GO:0005794; C:Golgi apparatus; ISS:UniProtKB.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0016020; C:membrane; IDA:MGI.
 GO:0004190; F:aspartic-type endopeptidase activity; ISS:UniProtKB.
 GO:0050435; P:beta-amyloid metabolic process; ISS:UniProtKB.
 GO:0006508; P:proteolysis; ISS:UniProtKB. 
Interpro
 IPR009119; Pept_A1_BACE.
 IPR009120; Pept_A1_BACE1.
 IPR001461; Peptidase_A1.
 IPR021109; Peptidase_aspartic.
 IPR001969; Peptidase_aspartic_AS. 
Pfam
 PF00026; Asp 
SMART
  
PROSITE
 PS00141; ASP_PROTEASE 
PRINTS
 PR01816; BACE1.
 PR01815; BACEFAMILY.
 PR00792; PEPSIN.