UniProt Accession

 UCHL4_MOUSE; P58321 

Genbank Protein ID

 AB035420 

Genbank Nucleotide ID

 BAB47122.1 

Protein Name

 Ubiquitin carboxyl-terminal hydrolase isozyme L4 

Protein Synonyms/Alias

 UCH-L4; Ubiquitin thioesterase L4 

Gene Name

 Uchl4 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
74	TEEEEKIKSQGQDVT	ubiquitination	[1]
124	ESGSTLKKFLEESVS	acetylation	[2]
124	ESGSTLKKFLEESVS	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 

Functional Description

 Ubiquitin-protein hydrolase is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. 

Sequence Annotation

 REGION 8 13 Interaction with ubiquitin (By
 REGION 222 227 Interaction with ubiquitin (By
 ACT_SITE 95 95 Nucleophile (By similarity).
 ACT_SITE 172 172 Proton donor (By similarity).
 MOD_RES 133 133 Phosphoserine.  

Keyword

 Complete proteome; Cytoplasm; Hydrolase; Phosphoprotein; Protease; Reference proteome; Thiol protease; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 233 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0008234; F:cysteine-type peptidase activity; IEA:UniProtKB-KW.
 GO:0004221; F:ubiquitin thiolesterase activity; IEA:InterPro.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro. 

Interpro

 IPR001578; Peptidase_C12. 

Pfam

 PF01088; Peptidase_C12 

SMART

  

PROSITE

 PS00140; UCH_1 

PRINTS

 PR00707; UBCTHYDRLASE.