CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001691
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Probable tRNA pseudouridine synthase 2 
Protein Synonyms/Alias
  
Gene Name
 TRUB2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
36TVELQLLKGLNARKPubiquitination[1, 2]
136TVRGLLGKATDDFREubiquitination[3]
296QVAAELEKSLSPGLDubiquitination[4]
305LSPGLDTKQLPSPGWubiquitination[4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 May be responsible for synthesis of pseudouridine from uracil in transfer RNAs (By similarity). 
Sequence Annotation
 ACT_SITE 98 98 Nucleophile (By similarity).  
Keyword
 Complete proteome; Isomerase; Polymorphism; Reference proteome; tRNA processing. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 331 AA 
Protein Sequence
MGSAGLSRLH GLFAVYKPPG LKWKHLRDTV ELQLLKGLNA RKPPAPKQRV RFLLGPMEGS 60
EEKELTLTAT SVPSFINHPL VCGPAFAHLK VGVGHRLDAQ ASGVLVLGVG HGCRLLTDMY 120
NAHLTKDYTV RGLLGKATDD FREDGRLVEK TTYDHVTREK LDRILAVIQG SHQKALVMYS 180
NLDLKTQEAY EMAVRGLIRP MNKSPMLITG IRCLYFAPPE FLLEVQCMHE TQKELRKLVH 240
EIGLELKTTA VCTQVRRTRD GFFTLDSALL RTQWDLTNIQ DAIRAATPQV AAELEKSLSP 300
GLDTKQLPSP GWSWDSQGPS STLGLERGAG Q 331 
Gene Ontology
 GO:0009982; F:pseudouridine synthase activity; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:InterPro.
 GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
 GO:0008033; P:tRNA processing; IEA:UniProtKB-KW. 
Interpro
 IPR002501; PsdUridine_synth.
 IPR020103; PsdUridine_synth_cat_dom. 
Pfam
 PF01509; TruB_N 
SMART
  
PROSITE
  
PRINTS