CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009080
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Hormonally up-regulated neu tumor-associated kinase 
Protein Synonyms/Alias
 B19; Serine/threonine-protein kinase MAK-V 
Gene Name
 HUNK 
Gene Synonyms/Alias
 MAKV 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
67NYLIGSRKLGEGSFAubiquitination[1]
75LGEGSFAKVREGLHVubiquitination[1]
91TGEKVAIKVIDKKRAubiquitination[1]
106KKDTYVTKNLRREGQubiquitination[1]
188GVVHRDLKIENLLLDubiquitination[1]
307LLEPDPVKRPNIQQAubiquitination[1]
327LNENYTGKVPCNVTYubiquitination[1]
392LERYLSGKSDIQDSLubiquitination[1]
402IQDSLCYKTRLYQIEubiquitination[1]
483QNTKALLKDRKASKSubiquitination[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
  
Sequence Annotation
 DOMAIN 62 320 Protein kinase.
 NP_BIND 68 76 ATP (By similarity).
 ACT_SITE 186 186 Proton acceptor (By similarity).
 BINDING 91 91 ATP (By similarity).
 CROSSLNK 483 483 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 ATP-binding; Complete proteome; Isopeptide bond; Kinase; Nucleotide-binding; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 714 AA 
Protein Sequence
MPAAAGDGLL GEPAAPGGGG GAEDAARPAA ACEGSFLPAW VSGVPRERLR DFQHHKRVGN 60
YLIGSRKLGE GSFAKVREGL HVLTGEKVAI KVIDKKRAKK DTYVTKNLRR EGQIQQMIRH 120
PNITQLLDIL ETENSYYLVM ELCPGGNLMH KIYEKKRLEE SEARRYIRQL ISAVEHLHRA 180
GVVHRDLKIE NLLLDEDNNI KLIDFGLSNC AGILGYSDPF STQCGSPAYA APELLARKKY 240
GPKIDVWSIG VNMYAMLTGT LPFTVEPFSL RALYQKMVDK EMNPLPTQLS TGAISFLRSL 300
LEPDPVKRPN IQQALANRWL NENYTGKVPC NVTYPNRISL EDLSPSVVLH MTEKLGYKNS 360
DVINTVLSNR ACHILAIYFL LNKKLERYLS GKSDIQDSLC YKTRLYQIEK YRAPKESYEA 420
SLDTWTRDLE FHAVQDKKPK EQEKRGDFLH RPFSKKLDKN LPSHKQPSGS LMTQIQNTKA 480
LLKDRKASKS SFPDKDSFGC RNIFRKTSDS NCVASSSMEF IPVPPPRTPR IVKKPEPHQP 540
GPGSTGIPHK EDPLMLDMVR SFESVDRDDH VEVLSPSHHY RILNSPVSLA RRNSSERTLS 600
PGLPSGSMSP LHTPLHPTLV SFAHEDKNSP PKEEGLCCPP PVPSNGPMQP LGSPNCVKSR 660
GRFPMMGIGQ MLRKRHQSLQ PSADRPLEAS LPPLQPLAPV NLAFDMADGV KTQC 714 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; TAS:ProtInc.
 GO:0007275; P:multicellular organismal development; TAS:ProtInc.
 GO:0007165; P:signal transduction; TAS:ProtInc. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
 SM00220; S_TKc 
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS