CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015800
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Phosphatidylinositol-binding clathrin assembly protein 
Protein Synonyms/Alias
 Clathrin assembly lymphoid myeloid leukemia; CALM 
Gene Name
 Picalm 
Gene Synonyms/Alias
 Calm; Fit1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
24VTGSAVSKTVCKATTubiquitination[1]
134YSRYLNEKAVSYRQVubiquitination[1]
163MRTMNTEKLLKTVPIubiquitination[1]
252TRISEFLKVAEQVGIubiquitination[1]
318STGLSLTKVDEREKQubiquitination[1]
324TKVDEREKQAALEEEubiquitination[1]
570SWSQPGEKKLTGGSNubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Assembly protein recruiting clathrin and adapter protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. Plays a crucial role in fetal and adult hematopoiesis, and normal prenatal and postnatal growth and viability. 
Sequence Annotation
 DOMAIN 14 145 ENTH.
 REGION 221 294 Interaction with FAM64A (By similarity).
 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 443 443 Phosphoserine.  
Keyword
 Acetylation; Alternative splicing; Coated pit; Complete proteome; Cytoplasmic vesicle; Developmental protein; Endocytosis; Golgi apparatus; Membrane; Nucleus; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 660 AA 
Protein Sequence
MSGQSLTDRI TAAQHSVTGS AVSKTVCKAT THEIMGPKKK HLDYLIQCTN EMNVNIPQLA 60
DSLFERTTNS SWVVVFKSLI TTHHLMVYGN ERFIQYLASR NTLFNLSNFL DKSGLQGYDM 120
STFIRRYSRY LNEKAVSYRQ VAFDFTKVKR GADGVMRTMN TEKLLKTVPI IQNQMDALLD 180
FNVNSNELTN GVINAAFMLL FKDAIRLFAA YNEGIINLLE KYFDMKKNQC KEGLDIYKKF 240
LTRMTRISEF LKVAEQVGID RGDIPDLSQA PSSLLDALEQ HLASLEGKKI KDSTAASRAT 300
TLSNAVSSLA STGLSLTKVD EREKQAALEE EQARLKALKE QRLKELAKKP HTSLTTAASP 360
VSTSAGGIMT APAIDIFSTP SSSNSTSKLP NDLLDLQQPT FHPSVHAMSA APQGASTWGD 420
PFSATLDAVE DAIPSLNPFL TKSSGDVHLP IASDVSTFTT RTPTHEMFVG FSPSPVAQPH 480
SSAGLNVDFE SVFGNKSTNV AVDSGGFDEL GGLLKPTVAS QNQSLPVAKL PPNKLVSDDL 540
DSSLANLVGN LGIGNGTTKN DVSWSQPGEK KLTGGSNWQP KVAPTTAWSA ATMNGMHFPQ 600
YAPPVMAYPA TTPTGMIGYG IPPQMGSVPV MTQPTLIYSQ PVMRPPNPFG PVSGAQIQFM 660 
Gene Ontology
 GO:0030122; C:AP-2 adaptor complex; IEA:Compara.
 GO:0005905; C:coated pit; IDA:UniProtKB.
 GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
 GO:0005622; C:intracellular; IDA:BHF-UCL.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005545; F:1-phosphatidylinositol binding; ISS:UniProtKB.
 GO:0030276; F:clathrin binding; ISS:UniProtKB.
 GO:0007409; P:axonogenesis; IMP:BHF-UCL.
 GO:0048268; P:clathrin coat assembly; IEA:InterPro.
 GO:0048813; P:dendrite morphogenesis; IMP:BHF-UCL.
 GO:0016197; P:endosomal transport; IEA:Compara.
 GO:0030097; P:hemopoiesis; IMP:UniProtKB.
 GO:0048261; P:negative regulation of receptor-mediated endocytosis; IEA:Compara.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IEA:Compara.
 GO:0031623; P:receptor internalization; IEA:Compara.
 GO:0006898; P:receptor-mediated endocytosis; IDA:UniProtKB.
 GO:0032880; P:regulation of protein localization; IEA:Compara. 
Interpro
 IPR011417; ANTH_dom.
 IPR014712; Clathrin_Pinositid-bd_GAT-like.
 IPR008942; ENTH_VHS.
 IPR013809; Epsin-like_N. 
Pfam
 PF07651; ANTH 
SMART
 SM00273; ENTH 
PROSITE
 PS50942; ENTH 
PRINTS