CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-041935
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 L-lactate dehydrogenase 
Protein Synonyms/Alias
  
Gene Name
 Ldha 
Gene Synonyms/Alias
 mCG_19938 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
34KSKMATLKDQLIVNLubiquitination[1]
43QLIVNLLKEEQAPQNubiquitination[1]
86LVDVMEDKLKGEMMDubiquitination[1]
88DVMEDKLKGEMMDLQubiquitination[1]
102QHGSLFLKTPKIVSSubiquitination[1]
105SLFLKTPKIVSSKDYubiquitination[1]
110TPKIVSSKDYCVTANubiquitination[1]
147QRNVNIFKFIIPNIVubiquitination[1]
155FIIPNIVKYSPHCKLubiquitination[1]
253ELGTDADKEQWKEVHubiquitination[1]
257DADKEQWKEVHKQVVubiquitination[1]
261EQWKEVHKQVVDSAYacetylation[2, 3, 4]
261EQWKEVHKQVVDSAYsuccinylation[4]
261EQWKEVHKQVVDSAYubiquitination[1]
272DSAYEVIKLKGYTSWacetylation[2, 3, 4, 5]
272DSAYEVIKLKGYTSWsuccinylation[4]
272DSAYEVIKLKGYTSWubiquitination[1]
347EEEARLKKSADTLWGacetylation[3, 4]
347EEEARLKKSADTLWGsuccinylation[4]
347EEEARLKKSADTLWGubiquitination[1]
357DTLWGIQKELQF***acetylation[3]
357DTLWGIQKELQF***ubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [3] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [5] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Glycolysis; NAD; Oxidoreductase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 361 AA 
Protein Sequence
MSKSSGGYTY TETSVLFFHF KVSKDSKSKM ATLKDQLIVN LLKEEQAPQN KITVVGVGAV 60
GMACAISILM KDLADELALV DVMEDKLKGE MMDLQHGSLF LKTPKIVSSK DYCVTANSKL 120
VIITAGARQQ EGESRLNLVQ RNVNIFKFII PNIVKYSPHC KLLIVSNPVD ILTYVAWKIS 180
GFPKNRVIGS GCNLDSARFR YLMGERLGVH ALSCHGWVLG EHGDSSVPVW SGVNVAGVSL 240
KSLNPELGTD ADKEQWKEVH KQVVDSAYEV IKLKGYTSWA IGLSVADLAE SIMKNLRRVH 300
PISTMIKGLY GINEDVFLSV PCILGQNGIS DVVKVTLTPE EEARLKKSAD TLWGIQKELQ 360
F 361 
Gene Ontology
 GO:0005929; C:cilium; IDA:MGI.
 GO:0005829; C:cytosol; IDA:MGI.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0004459; F:L-lactate dehydrogenase activity; IDA:MGI.
 GO:0044262; P:cellular carbohydrate metabolic process; IEA:InterPro.
 GO:0031668; P:cellular response to extracellular stimulus; IDA:MGI.
 GO:0006096; P:glycolysis; IEA:UniProtKB-KW. 
Interpro
 IPR001557; L-lactate/malate_DH.
 IPR011304; L-lactate_DH.
 IPR018177; L-lactate_DH_AS.
 IPR022383; Lactate/malate_DH_C.
 IPR001236; Lactate/malate_DH_N.
 IPR015955; Lactate_DH/Glyco_Ohase_4_C.
 IPR016040; NAD(P)-bd_dom. 
Pfam
 PF02866; Ldh_1_C
 PF00056; Ldh_1_N 
SMART
  
PROSITE
 PS00064; L_LDH 
PRINTS
 PR00086; LLDHDRGNASE.