CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018874
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 G patch domain and KOW motifs-containing protein 
Protein Synonyms/Alias
 G patch domain-containing protein 5; Protein MOS2 homolog; Protein T54 
Gene Name
 GPKOW 
Gene Synonyms/Alias
 GPATC5; GPATCH5; T54 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
178MLRGMGWKPGEGIGRacetylation[1, 2]
192RTFNQVVKPRVNSLRacetylation[3]
192RTFNQVVKPRVNSLRubiquitination[4, 5]
201RVNSLRPKGLGLGANubiquitination[6]
299VSQQEFDKNTLDLRQubiquitination[3, 7]
315NGTASSRKTLWNQELubiquitination[4, 5]
346RQDGPAAKSEKAAPRacetylation[3]
372RFVDNMYKGGQYYNTubiquitination[4, 5, 7]
Reference
 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [7] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961
Functional Description
  
Sequence Annotation
 DOMAIN 164 210 G-patch.
 DOMAIN 240 267 KOW 1.
 DOMAIN 415 442 KOW 2.
 MOD_RES 42 42 Phosphoserine.
 MOD_RES 216 216 Phosphothreonine.  
Keyword
 Complete proteome; Nucleus; Phosphoprotein; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 476 AA 
Protein Sequence
MADSKEGVLP LTAASTAPIS FGFTRTSARR RLADSGDGAG PSPEEKDFLK TVEGRELQSV 60
KPQEAPKELV IPLIQNGHRR QPPARPPGPS TDTGALADGV VSQAVKELIA ESKKSLEERE 120
NAGVDPTLAI PMIQKGCTPS GEGADSEPRA ETVPEEANYE AVPVEAYGLA MLRGMGWKPG 180
EGIGRTFNQV VKPRVNSLRP KGLGLGANLT EAQALTPTGP SRMPRPDEEQ EKDKEDQPQG 240
LVPGGAVVVL SGPHRGLYGK VEGLDPDNVR AMVRLAVGSR VVTVSEYYLR PVSQQEFDKN 300
TLDLRQQNGT ASSRKTLWNQ ELYIQQDNSE RKRKHLPDRQ DGPAAKSEKA APRSQHWLHR 360
DLRVRFVDNM YKGGQYYNTK MIIEDVLSPD TCVCRTDEGR VLEGLREDML ETLVPKAEGD 420
RVMVVLGPQT GRVGHLLSRD RARSRALVQL PRENQVVELH YDAICQYMGP SDTDDD 476 
Gene Ontology
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0003676; F:nucleic acid binding; IEA:InterPro. 
Interpro
 IPR000467; G_patch_dom.
 IPR005824; KOW. 
Pfam
 PF00467; KOW 
SMART
 SM00443; G_patch
 SM00739; KOW 
PROSITE
 PS50174; G_PATCH 
PRINTS