CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-037603
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Calpain-1 catalytic subunit 
Protein Synonyms/Alias
  
Gene Name
 CAPN1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
71VPQSLGYKDLGPNSSubiquitination[1]
79DLGPNSSKTYGIKWKubiquitination[1, 2, 3]
84SSKTYGIKWKRPTELubiquitination[1, 2, 3, 4]
86KTYGIKWKRPTELLSubiquitination[2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Hydrolase; Protease; Reference proteome; Thiol protease. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 162 AA 
Protein Sequence
MSEEIITPVY CTGVSAQVQK QRARELGLGR HENAIKYLGQ DYEQLRVRCL QSGTLFRDEA 60
FPPVPQSLGY KDLGPNSSKT YGIKWKRPTE LLSNPQFIVD GATRTDICQG ALGDCWLLAA 120
IASLTLNDTL LHRVVPHGQS FQNGYAGIFH FQLWQFGEWV DV 162 
Gene Ontology
 GO:0005622; C:intracellular; IEA:InterPro.
 GO:0004198; F:calcium-dependent cysteine-type endopeptidase activity; IEA:InterPro.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
 GO:0032801; P:receptor catabolic process; IEA:Compara. 
Interpro
 IPR022684; Calpain_cysteine_protease.
 IPR000169; Pept_cys_AS.
 IPR001300; Peptidase_C2_calpain_cat. 
Pfam
 PF00648; Peptidase_C2 
SMART
 SM00230; CysPc 
PROSITE
 PS50203; CALPAIN_CAT
 PS00139; THIOL_PROTEASE_CYS 
PRINTS
 PR00704; CALPAIN.