CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001321
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA fragmentation factor subunit beta 
Protein Synonyms/Alias
 Caspase-activated deoxyribonuclease; CAD; Caspase-activated DNase; Caspase-activated nuclease; CPAN; DNA fragmentation factor 40 kDa subunit; DFF-40 
Gene Name
 DFFB 
Gene Synonyms/Alias
 CAD; DFF2; DFF40 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
152LESRFQSKSGYLRYSubiquitination[1, 2]
264NLDHIIEKKRTIIPTubiquitination[1, 2, 3, 4, 5, 6]
265LDHIIEKKRTIIPTLubiquitination[2, 6]
277PTLVEAIKEQDGREVubiquitination[2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology. 
Sequence Annotation
 DOMAIN 4 80 CIDE-N.  
Keyword
 3D-structure; Alternative splicing; Apoptosis; Complete proteome; Cytoplasm; Hydrolase; Nuclease; Nucleus; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 338 AA 
Protein Sequence
MLQKPKSVKL RALRSPRKFG VAGRSCQEVL RKGCLRFQLP ERGSRLCLYE DGTELTEDYF 60
PSVPDNAELV LLTLGQAWQG YVSDIRRFLS AFHEPQVGLI QAAQQLLCDE QAPQRQRLLA 120
DLLHNVSQNI AAETRAEDPP WFEGLESRFQ SKSGYLRYSC ESRIRSYLRE VSSYPSTVGA 180
EAQEEFLRVL GSMCQRLRSM QYNGSYFDRG AKGGSRLCTP EGWFSCQGPF DMDSCLSRHS 240
INPYSNRESR ILFSTWNLDH IIEKKRTIIP TLVEAIKEQD GREVDWEYFY GLLFTSENLK 300
LVHIVCHKKT THKLNCDPSR IYKPQTRLKR KQPVRKRQ 338 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0000790; C:nuclear chromatin; IDA:UniProtKB.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0004536; F:deoxyribonuclease activity; TAS:ProtInc.
 GO:0003677; F:DNA binding; IEA:Compara.
 GO:0004516; F:nicotinate phosphoribosyltransferase activity; IEA:Compara.
 GO:0030263; P:apoptotic chromosome condensation; IDA:UniProtKB.
 GO:0006309; P:apoptotic DNA fragmentation; IDA:MGI.
 GO:0006917; P:induction of apoptosis; IEA:Compara.
 GO:0035556; P:intracellular signal transduction; TAS:ProtInc. 
Interpro
 IPR015311; Apoptosis_DFF40.
 IPR003508; CAD. 
Pfam
 PF02017; CIDE-N
 PF09230; DFF40 
SMART
  
PROSITE
 PS51135; CIDE_N 
PRINTS