CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024309
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Putative PIP5K1A and PSMD4-like protein 
Protein Synonyms/Alias
 PIP5K1A-PSMD4 
Gene Name
 PIPSL 
Gene Synonyms/Alias
 PSMD4P2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
87HYNAFRFKTYAPVAFubiquitination[1]
559DTGRILSKLHTVQPKubiquitination[1, 2, 3, 4, 5, 6, 7, 8]
566KLHTVQPKGKITFCMubiquitination[1, 2, 3, 4, 6]
620AKCLKKEKVNVDIINubiquitination[1, 2, 3, 4, 6, 8]
637EEEVNTEKLTAFVNTubiquitination[1, 3, 4, 6, 7, 8]
747DSDDALLKMTISQQEubiquitination[7]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [8] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
 Has negligeable PIP5 kinase activity. Binds to ubiquitinated proteins. 
Sequence Annotation
 DOMAIN 28 396 PIPK.
 DOMAIN 490 673 VWFA.
 REPEAT 696 715 UIM 1.
 REPEAT 766 783 UIM 2.  
Keyword
 Complete proteome; Cytoplasm; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 862 AA 
Protein Sequence
MASEVPYASG MPIKKIGHRS VDSSGGTTSS ALKGAIQLGI THTVGSLSTK PESDVLMQDF 60
HMVESIFFPS EGSNLTPAHH YNAFRFKTYA PVAFRYFWEL FGIRPDDYLY SLCSEPLIEL 120
CSSGASGSLF YVSSDDEFIV KTVRHKEAEF LQKLLPGYYI NLNQNPRTLL PKFYGLYCVQ 180
TGGKNIRIVV MNNLLPRSVK MHIKYDLKGS TYRRRASQKE REKPLPTFKD LDFLQDIPDG 240
LFLDADVHNA LCKTLQRDCL VLQSFKIMDY SLLMSIHNID HAQREPLSSE TQYSVDTRRP 300
APQKALYSTA MESIQGEARR GGTMETDDHM GGIPARNSKG ERLLLYIGII DILQSYRFVK 360
KLEHSWKALI HDGDTVSVHR PGFYAEWFQR FMCNTVFKKI PLKPSPSKKL RSGSSFSQRA 420
GSSGNSCITY QPLVSGEHKA QVTTKAEVEP GVHLGCPDVL PQTPPLEEIS EGSPTPDPSF 480
SPLVEETLQM LTTSVDNSEY MGNGDFLPTR LQAQQDAVNT VCHSKTRSNP ENNVGLITLD 540
NDCEVLTTLT PDTGRILSKL HTVQPKGKIT FCMGIHVAHL ALKHRQGNNH KIRIIAFVGN 600
PVEDNEKNLV KLAKCLKKEK VNVDIINFGE EEVNTEKLTA FVNTLNGKDG TGSHLVTVPP 660
GPSLADALIS FPILAGEGGA MMGLGASDFE FGVDPSADPE LALVLRVFME EQRQRQEEEA 720
RQAAAASAAE AGIATTGTED SDDALLKMTI SQQEFGHTGL PDLSSMTEEE KIVCAMQMSL 780
QGAEFGLAES ADIDASSAMD TSEPAKEEDD YDVMQDPEFL QSVLENLPGV DPNNEAIRNA 840
VGSLASQATK DSKKDKKEED KK 862 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0016307; F:phosphatidylinositol phosphate kinase activity; IEA:InterPro.
 GO:0046854; P:phosphatidylinositol phosphorylation; IEA:GOC. 
Interpro
 IPR023610; PInositol-4-P-5-kinase.
 IPR027483; PInositol-4-P-5-kinase_C.
 IPR002498; PInositol-4-P-5-kinase_core.
 IPR027484; PInositol-4-P-5-kinase_N.
 IPR016034; PInositol-4P-5-kinase_core_sub.
 IPR002035; VWF_A. 
Pfam
 PF01504; PIP5K 
SMART
 SM00330; PIPKc
 SM00327; VWA 
PROSITE
 PS51455; PIPK
 PS50330; UIM
 PS50234; VWFA 
PRINTS