CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019012
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein TBRG4 
Protein Synonyms/Alias
 Cell cycle progression restoration protein 2; Cell cycle progression protein 2; FAST kinase domain-containing protein 4; Transforming growth factor beta regulator 4 
Gene Name
 TBRG4 
Gene Synonyms/Alias
 CPR2; FASTKD4; KIAA0948 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
70ASTPYIEKQVDHLIKubiquitination[1]
163LGIPKASKELQSVEQubiquitination[2, 3]
337GEVAHCAKSFALLKWacetylation[4]
494RKVTPLQKELQETLKubiquitination[3]
559QSPPPGSKRLAFLRWubiquitination[5]
623YLKDKMRKAVAEELAubiquitination[3]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 May play a role in cell cycle progression. 
Sequence Annotation
 DOMAIN 561 619 RAP.  
Keyword
 Alternative splicing; Complete proteome; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 631 AA 
Protein Sequence
MAAHLVKRCT CLLREAARQA PAMAPVGRLR LAWVAHKTLT SSATSPISHL PGSLMEPVEK 60
ERASTPYIEK QVDHLIKKAT RPEELLELLG GSHDLDSNQA AMVLIRLSHL LSEKPEDKGL 120
LIQDAHFHQL LCLLNSQIAS VWHGTLSKLL GSLYALGIPK ASKELQSVEQ EVRWRMRKLK 180
YKHLAFLAES CATLSQEQHS QELLAELLTH LERRWTEIED SHTLVTVMMK VGHLSEPLMN 240
RLEDKCLELV EHFGPNELRK VLVMLAAQSR RSVPLLRAIS YHLVQKPFSL TKDVLLDVAY 300
AYGKLSFHQT QVSQRLATDL LSLMPSLTSG EVAHCAKSFA LLKWLSLPLF EAFAQHVLNR 360
AQDITLPHLC SVLLAFARLN FHPDQEDQFF SLVHEKLGSE LPGLEPALQV DLVWALCVLQ 420
QAREAELQAV LHPEFHIQFL GGKSQKDQNT FQKLLHINAT ALLEYPEYSG PLLPASAVAP 480
GPSALDRKVT PLQKELQETL KGLLGSADKG SLEVATQYGW VLDAEVLLDS DGEFLPVRDF 540
VAPHLAQPTG SQSPPPGSKR LAFLRWEFPN FNSRSKDLLG RFVLARRHIV AAGFLIVDVP 600
FYEWLELKSE WQKGAYLKDK MRKAVAEELA K 631 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:UniProtKB.
 GO:0004672; F:protein kinase activity; IEA:InterPro.
 GO:0007050; P:cell cycle arrest; TAS:UniProtKB.
 GO:0045333; P:cellular respiration; ISS:UniProtKB.
 GO:0000080; P:G1 phase of mitotic cell cycle; TAS:UniProtKB.
 GO:0008284; P:positive regulation of cell proliferation; TAS:UniProtKB.
 GO:0006468; P:protein phosphorylation; IEA:GOC. 
Interpro
 IPR013579; FAST_2.
 IPR010622; FAST_Leu-rich.
 IPR013584; RAP. 
Pfam
 PF06743; FAST_1
 PF08368; FAST_2
 PF08373; RAP 
SMART
 SM00952; RAP 
PROSITE
 PS51286; RAP 
PRINTS