UniProt Accession

 KKCC2_HUMAN; Q96RR4; A8K7Q7; O94883; Q8IUG2; Q8IUG3; Q8N3I4; Q8WY03; Q8WY04; Q8WY05; Q8WY06; Q96RP1; Q96RP2; Q96RR3; Q9BWE9; Q9UER3; Q9UES2; Q9Y5N2 

Genbank Protein ID

 AB081337; AF287630; AF287631; AF321385; AF321386; AF321387; AF321388; AF321401; AF321390; AF321391; AF321392; AF321393; AF321394; AF321395; AF321396; AF321397; AF321398; AF321399; AF321400; AF321575; AF321576; AF321577; AF321578; AF321402; AF321390; AF321391; AF321392; AF321393; AF321394; AF321395; AF321396; AF321397; AF321398; AF321399; AF321400; AF321575; AF321576; AF321577; AF321578; AF140507; AB081336; AB018330; AK292072; AC069209; BC000318; BC026060; AF101264; AL834322; AF091074 

Genbank Nucleotide ID

 BAC19841.1; AAK64600.1; AAK64601.1; AAL37215.1; AAL37216.1; AAL37217.1; AAL37218.1; AAK91830.1; AAK91830.1; AAK91830.1; AAK91830.1; AAK91830.1; AAK91830.1; AAK91830.1; AAK91830.1; AAK91830.1; AAK91830.1; AAK91830.1; AAK91830.1; AAK91830.1; AAK91830.1; AAK91830.1; AAK91830.1; AAK91829.1; AAK91829.1; AAK91829.1; AAK91829.1; AAK91829.1; AAK91829.1; AAK91829.1; AAK91829.1; AAK91829.1; AAK91829.1; AAK91829.1; AAK91829.1; AAK91829.1; AAK91829.1; AAK91829.1; AAK91829.1; AAD31507.1; BAC19840.1; BAA34507.2; BAF84761.1; AAH00318.2; AAH26060.1; AAD04566.1; CAD38990.1; AAC72943.1 

Protein Name

 Calcium/calmodulin-dependent protein kinase kinase 2 

Protein Synonyms/Alias

 CaM-KK 2; CaM-kinase kinase 2; CaMKK 2; Calcium/calmodulin-dependent protein kinase kinase beta; CaM-KK beta; CaM-kinase kinase beta; CaMKK beta 

Gene Name

 CAMKK2 

Gene Synonyms/Alias

 CAMKKB; KIAA0787 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
32	SSSSESQKPCEALRG	ubiquitination	[1]
98	RPHLSGRKLSLQERS	ubiquitination	[1]
194	DNTYYAMKVLSKKKL	ubiquitination	[1]
241	YQEIAILKKLDHPNV	ubiquitination	[1]
242	QEIAILKKLDHPNVV	ubiquitination	[1]
307	IEYLHYQKIIHRDIK	ubiquitination	[1]
401	RIMCLHSKIKSQALE	ubiquitination	[1]
403	MCLHSKIKSQALEFP	ubiquitination	[1]
429	LITRMLDKNPESRIV	ubiquitination	[1]
441	RIVVPEIKLHPWVTR	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin- binding domain are inactive. Efficiently phosphorylates 5'-AMP- activated protein kinase (AMPK) trimer, including that consisting of PRKAA1, PRKAB1 and PRKAG1. This phosphorylation is stimulated in response to Ca(2+) signals (By similarity). Seems to be involved in hippocampal activation of CREB1 (By similarity). May play a role in neurite growth. Isoform 3 may promote neurite elongation, while isoform 1 may promoter neurite branching. 

Sequence Annotation

 DOMAIN 165 446 Protein kinase.
 NP_BIND 171 179 ATP (By similarity).
 REGION 204 226 RP domain.
 REGION 472 477 Autoinhibitory domain (By similarity).
 REGION 475 500 Calmodulin-binding (By similarity).
 ACT_SITE 312 312 Proton acceptor (By similarity).
 BINDING 194 194 ATP (By similarity).
 MOD_RES 129 129 Phosphoserine (By similarity).
 MOD_RES 133 133 Phosphoserine (By similarity).
 MOD_RES 495 495 Phosphoserine.  

Keyword

 3D-structure; Alternative splicing; ATP-binding; Calmodulin-binding; Cell projection; Complete proteome; Cytoplasm; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 588 AA 

Protein Sequence

Gene Ontology

 GO:0042995; C:cell projection; IEA:UniProtKB-SubCell.
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0005509; F:calcium ion binding; IDA:UniProtKB.
 GO:0005516; F:calmodulin binding; TAS:UniProtKB.
 GO:0004683; F:calmodulin-dependent protein kinase activity; IEA:EC.
 GO:0004713; F:protein tyrosine kinase activity; TAS:UniProtKB.
 GO:0019722; P:calcium-mediated signaling; TAS:UniProtKB.
 GO:0000165; P:MAPK cascade; TAS:UniProtKB.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; TAS:UniProtKB.
 GO:0046777; P:protein autophosphorylation; IDA:UniProtKB.
 GO:0045859; P:regulation of protein kinase activity; TAS:UniProtKB. 

Interpro

 IPR020636; Ca/CaM-dep_Ca-dep_prot_Kinase.
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 

Pfam

 PF00069; Pkinase 

SMART

 SM00220; S_TKc 

PROSITE

 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 

PRINTS