CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-039745
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Amyloid-like protein 2 
Protein Synonyms/Alias
  
Gene Name
 APLP2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
428VLAVDPEKAAQMKSQubiquitination[1]
639PEERHLNKMQNHGYEubiquitination[1, 2, 3, 4, 5, 6]
651GYENPTYKYLEQMQIubiquitination[1, 2, 3, 4, 5]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 658 AA 
Protein Sequence
MYPELQITNV MEANQRVSID NWCRRDKKQC KSRFVTPFKC LVGEFVSDVL LVPEKCQFFH 60
KERMEVCENH QHWHTVVKEA CLTQGMTLYS YGMLLPCGVD QFHGTEYVCC PQTKIIGSVS 120
KEEEEEDEEE EEEEDEEEDY DVYKSEFPTE ADLEDFTEAA VDEDDEDEEE GEEVVEDRDY 180
YYDTFKGDDY NEENPTEPGS DGTMSDKEIT HDVKAVCSQE AMTGPCRAVM PRWYFDLSKG 240
KCVRFIYGGC GGNRNNFESE DYCMAVCKAM IPPTPLPTND VDVYFETSAD DNEHARFQKA 300
KEQLEIRHRN RMDRVKKEWE EAELQAKNLP KAERQTLIQH FQAMVKALEK EAASEKQQLV 360
ETHLARVEAM LNDRRRMALE NYLAALQSDP PRPHRILQAL RRYVRAENKD RLHTIRHYQH 420
VLAVDPEKAA QMKSQVMTHL HVIEERRNQS LSLLYKVPYV AQEIQEEIDE LLQEQRADMD 480
QFTASISETP VDVRVSSEES EEIPPFHPFH PFPALPENEG SGVGEQDGGL IGAEEKVINS 540
KNKVDENMVI DETLDVKEMI FNAERVGGLE EERESVGPLR EDFSLSSSAL IGLLVIAVAI 600
ATVIVISLVM LRKRQYGTIS HGIVEVDPML TPEERHLNKM QNHGYENPTY KYLEQMQI 658 
Gene Ontology
 GO:0016021; C:integral to membrane; IEA:InterPro.
 GO:0008201; F:heparin binding; IEA:InterPro.
 GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:InterPro.
 GO:0046914; F:transition metal ion binding; IEA:InterPro. 
Interpro
 IPR008155; Amyloid_glyco.
 IPR011178; Amyloid_glyco_Cu-bd.
 IPR024329; Amyloid_glyco_E2_domain.
 IPR019744; Amyloid_glyco_extracell_CS.
 IPR015849; Amyloid_glyco_heparin-bd.
 IPR019745; Amyloid_glyco_intracell_CS.
 IPR019543; APP_amyloid_C.
 IPR002223; Prot_inh_Kunz-m.
 IPR020901; Prtase_inh_Kunz-CS. 
Pfam
 PF10515; APP_amyloid
 PF12924; APP_Cu_bd
 PF12925; APP_E2
 PF02177; APP_N
 PF00014; Kunitz_BPTI 
SMART
 SM00131; KU 
PROSITE
 PS00319; A4_EXTRA
 PS00320; A4_INTRA
 PS00280; BPTI_KUNITZ_1
 PS50279; BPTI_KUNITZ_2 
PRINTS
 PR00203; AMYLOIDA4.
 PR00759; BASICPTASE.