CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-044154
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Hepatocyte growth factor-regulated tyrosine kinase substrate 
Protein Synonyms/Alias
  
Gene Name
 HGS 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
56IKKKVNDKNPHVALYubiquitination[1, 2, 3]
120QDTYQIMKVEGHVFPubiquitination[1, 3, 4, 5, 6]
130GHVFPEFKESDAMFAubiquitination[1, 3, 5]
176CGQIFCGKCSSKYSTubiquitination[1, 2, 5, 6, 7]
180FCGKCSSKYSTIPKFubiquitination[1, 3]
186SKYSTIPKFGIEKEVubiquitination[1, 2, 3, 4, 6, 7]
191IPKFGIEKEVRVCEPacetylation[6]
191IPKFGIEKEVRVCEPubiquitination[1, 2, 3, 4, 5, 6, 7]
206CYEQLNRKAEGKATSubiquitination[1, 3]
210LNRKAEGKATSTTELubiquitination[1, 3]
235QQSQLPPKRDETALQubiquitination[1, 3, 4, 5]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Metal-binding; Reference proteome; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 263 AA 
Protein Sequence
MGRGSGTFER LLDKATSQLL LETDWESILQ ICDLIRQGDT QAKYAVNSIK KKVNDKNPHV 60
ALYALEVMES VVKNCGQTVH DEVANKQTME ELKDLLKAWA HAFRNEPKYK VVQDTYQIMK 120
VEGHVFPEFK ESDAMFAAER APDWVDAEEC HRCRVQFGVM TRKHHCRACG QIFCGKCSSK 180
YSTIPKFGIE KEVRVCEPCY EQLNRKAEGK ATSTTELPPE YLTSPLSQQS QLPPKRDETA 240
LQEEEELQLA LALSQSEAEE KER 263 
Gene Ontology
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0016197; P:endosomal transport; IEA:InterPro.
 GO:0006886; P:intracellular protein transport; IEA:InterPro.
 GO:0009966; P:regulation of signal transduction; IEA:InterPro. 
Interpro
 IPR008942; ENTH_VHS.
 IPR027426; HGS.
 IPR003903; Ubiquitin-int_motif.
 IPR002014; VHS.
 IPR018205; VHS_subgr.
 IPR000306; Znf_FYVE.
 IPR017455; Znf_FYVE-rel.
 IPR011011; Znf_FYVE_PHD.
 IPR013083; Znf_RING/FYVE/PHD. 
Pfam
 PF01363; FYVE
 PF00790; VHS 
SMART
 SM00064; FYVE
 SM00726; UIM
 SM00288; VHS 
PROSITE
 PS50330; UIM
 PS50179; VHS
 PS50178; ZF_FYVE 
PRINTS