CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-036950
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 WASH complex subunit strumpellin 
Protein Synonyms/Alias
  
Gene Name
 KIAA0196 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
163WEPYKAAKTALNNTLubiquitination[1]
198AQVQQFLKEGYLREEubiquitination[2, 3, 4]
242SACDPNNKRLRQIKDubiquitination[3]
313ADVFSGVKPLTRVEKubiquitination[1, 3, 4, 5]
320KPLTRVEKNENLQAWubiquitination[3]
333AWFREISKQILSLNYubiquitination[1]
433VNPSMVTKLRATFLKubiquitination[1]
440KLRATFLKLASALDLubiquitination[1]
510EVPTRLDKDKLRDYAubiquitination[6]
512PTRLDKDKLRDYAQLubiquitination[6]
594SELMPKLKELGATMDubiquitination[1, 3, 5]
645CNNFLRTKIQDWQSMubiquitination[3]
826YSCRFDSKHLAAALEubiquitination[4, 5, 6]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1011 AA 
Protein Sequence
MLLVIDQKIE GEVRERMLVS YYRYSAARSS ADSNMDDICK LLRSTGYSSQ PGAKRPSNYP 60
ESYFQRVPIN ESFISMVIGR LRSDDIYNQV SAYPLPEHRS TALANQAAML YVILYFEPSI 120
LHTHQAKMRE IVDKYFPDNW VISIYMGITV NLVDAWEPYK AAKTALNNTL DLSNVREQAS 180
RYATVSERVH AQVQQFLKEG YLREEMVLDN IPKLLNCLRD CNVAIRWLML HTADSACDPN 240
NKRLRQIKDQ ILTDSRYNPR ILFQLLLDTA QFEFILKEMF KQMLSEKQTK WEHYKKEGSE 300
RMTELADVFS GVKPLTRVEK NENLQAWFRE ISKQILSLNY DDSTAAGRKT VQLIQALEEV 360
QEFHQLESNL QVCQFLADTR KFLHQMIRTI NIKEEVLITM QIVGDLSFAW QLIDSFTSIM 420
QESIRVNPSM VTKLRATFLK LASALDLPLL RINQANSPDL LSVSQYYSGE LVSYVRKVLQ 480
IIPESMFTSL LKIIKLQTHD IIEVPTRLDK DKLRDYAQLG PRYEVAKLTH AISIFTEGIL 540
MMKTTLVGII KVDPKQLLED GIRKELVKRV AFALHRGLIF NPRAKPSELM PKLKELGATM 600
DGFHRSFEYI QDYVNIYGLK IWQEEVSRII NYNVEQECNN FLRTKIQDWQ SMYQSTHIPI 660
PKFTPVDESV TFIGRLCREI LRITDPKMTC HIDQLNTWYD MKTHQEVTSS RLFSEIQTTL 720
GTFGLNGLDR LLCFMIVKEL QNFLSMFQKI ILRDRTVQDT LKTLMNAVSP LKSIVANSNK 780
IYFSAIAKTQ KIWTAYLEAI MKVGQMQILR QQIANELNYS CRFDSKHLAA ALENLNKALL 840
ADIEAHYQDP SLPYPKEDNT LLYEITAYLE AAGIHNPLNK IYITTKRLPY FPIVNFLFLI 900
AQLPKLQYNK NLGMVCRKPT DPVDWPPLVL GLLTLLKQFH SRYTEQFLAL IGQFICSTVE 960
QCTSQKIPEI PADVVGALLF LEDYVRYTKL PRRVAEAHVP NFIFDEFRTV L 1011 
Gene Ontology
 GO:0005768; C:endosome; IEA:Compara.
 GO:0071203; C:WASH complex; IEA:InterPro.
 GO:0016197; P:endosomal transport; IEA:Compara. 
Interpro
 IPR019393; WASH_strumpellin. 
Pfam
 PF10266; Strumpellin 
SMART
  
PROSITE
  
PRINTS