CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016018
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ubiquitin carboxyl-terminal hydrolase CYLD 
Protein Synonyms/Alias
 Deubiquitinating enzyme CYLD; Ubiquitin thioesterase CYLD; Ubiquitin-specific-processing protease CYLD 
Gene Name
 Cyld 
Gene Synonyms/Alias
 Cyld1; Kiaa0849 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
626VLLRPKEKNDIEYYSubiquitination[1]
720FYQIFMEKNEKVGVPubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Protease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Has endodeubiquitinase activity. Plays an important role in the regulation of pathways leading to NF-kappa-B activation. Contributes to the regulation of cell survival, proliferation and differentiation via its effects on NF-kappa-B activation. Negative regulator of Wnt signaling. Inhibits HDAC6 and thereby promotes acetylation of alpha-tubulin and stabilization of microtubules. Plays a role in the regulation of microtubule dynamics, and thereby contributes to the regulation of cell proliferation, cell polarization, cell migration, and angiogenesis. Required for normal cell cycle progress and normal cytokinesis. Inhibits nuclear translocation of NF-kappa-B (By similarity). Plays a role in the regulation of inflammation and the innate immune response, via its effects on NF-kappa-B activation. Dispensable for the maturation of intrathymic natural killer cells, but required for the continued survival of immature natural killer cells. Negatively regulates TNFRSF11A signaling and osteoclastogenesis. 
Sequence Annotation
 DOMAIN 153 198 CAP-Gly 1.
 DOMAIN 253 286 CAP-Gly 2.
 DOMAIN 488 531 CAP-Gly 3.
 REGION 106 589 Interaction with TRIP (By similarity).
 REGION 390 465 Interaction with TRAF2 (By similarity).
 REGION 466 680 Interaction with IKBKG/NEMO (By
 ACT_SITE 597 597 Nucleophile (By similarity).
 ACT_SITE 867 867 Proton acceptor (By similarity).
 METAL 784 784 Zinc 1 (By similarity).
 METAL 787 787 Zinc 1 (By similarity).
 METAL 795 795 Zinc 2 (By similarity).
 METAL 798 798 Zinc 2 (By similarity).
 METAL 813 813 Zinc 1 (By similarity).
 METAL 816 816 Zinc 1 (By similarity).
 METAL 821 821 Zinc 2 (By similarity).
 METAL 829 829 Zinc 2 (By similarity).
 MOD_RES 414 414 Phosphoserine.  
Keyword
 Alternative splicing; Cell membrane; Complete proteome; Cytoplasm; Cytoskeleton; Hydrolase; Membrane; Metal-binding; Microtubule; Phosphoprotein; Protease; Reference proteome; Repeat; Thiol protease; Ubl conjugation pathway; Wnt signaling pathway; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 952 AA 
Protein Sequence
MSSGLWSQEK VTSPYWEERI FYLLLQECSV TDKQTQKLLK VPKGSIGQYI QDRSVGHSRV 60
PSTKGKKNQI GLKILEQPHA VLFVDEKDVV EINEKFTELL LAITNCEERL SLFRNRLRLS 120
KGLQVDVGSP VKVQLRSGEE KFPGVVRFRG PLLAERTVSG IFFGVELLEE GRGQGFTDGV 180
YQGKQLFQCD EDCGVFVALD KLELIEDDDN GLESDFAGPG DTMQVEPPPL EINSRVSLKV 240
GESTESGTVI FCDVLPGKES LGYFVGVDMD NPIGNWDGRF DGVQLCSFAS VESTILLHIN 300
DIIPALSDSV TQERRPPKLA FMSRGVGDKG SSSHNKPKVT GSTSDPGSRN RSELFYTLNG 360
SSVDSQQSKS KNPWYIDEVA EDPAKSLTEM SSDFGHSSPP PQPPSMNSLS SENRFHSLPF 420
SLTKMPNTNG SMAHSPLSLS VQSVMGELNS TPVQESPPLP ISSGNAHGLE VGSLAEVKEN 480
PPFYGVIRWI GQPPGLSDVL AGLELEDECA GCTDGTFRGT RYFTCALKKA LFVKLKSCRP 540
DSRFASLQPV SNQIERCNSL AFGGYLSEVV EENTPPKMEK EGLEIMIGKK KGIQGHYNSC 600
YLDSTLFCLF AFSSALDTVL LRPKEKNDIE YYSETQELLR TEIVNPLRIY GYVCATKIMK 660
LRKILEKVEA ASGFTSEEKD PEEFLNILFH DILRVEPLLK IRSAGQKVQD CNFYQIFMEK 720
NEKVGVPTIQ QLLEWSFINS NLKFAEAPSC LIIQMPRFGK DFKLFKKIFP SLELNITDLL 780
EDTPRQCRIC GGLAMYECRE CYDDPDISAG KIKQFCKTCS TQVHLHPRRL NHSYHPVSLP 840
KDLPDWDWRH GCIPCQKMEL FAVLCIETSH YVAFVKYGKD DSAWLFFDSM ADRDGGQNGF 900
NIPQVTPCPE VGEYLKMSLE DLHSLDSRRI QGCARRLLCD AYMCMYQSPT MSLYK 955 
Gene Ontology
 GO:0005881; C:cytoplasmic microtubule; IEA:Compara.
 GO:0005829; C:cytosol; ISS:UniProtKB.
 GO:0031234; C:extrinsic to internal side of plasma membrane; IEA:Compara.
 GO:0030496; C:midbody; IEA:Compara.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0004221; F:ubiquitin thiolesterase activity; IEA:InterPro.
 GO:0004843; F:ubiquitin-specific protease activity; ISS:UniProtKB.
 GO:0008270; F:zinc ion binding; ISS:UniProtKB.
 GO:0006917; P:induction of apoptosis; IEA:Compara.
 GO:0090090; P:negative regulation of canonical Wnt receptor signaling pathway; ISS:UniProtKB.
 GO:0042347; P:negative regulation of NF-kappaB import into nucleus; ISS:UniProtKB.
 GO:0032088; P:negative regulation of NF-kappaB transcription factor activity; ISS:UniProtKB.
 GO:2001238; P:positive regulation of extrinsic apoptotic signaling pathway; IEA:Compara.
 GO:0070536; P:protein K63-linked deubiquitination; ISS:UniProtKB.
 GO:0070507; P:regulation of microtubule cytoskeleton organization; IEA:Compara.
 GO:0007346; P:regulation of mitotic cell cycle; IEA:Compara.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro.
 GO:0016055; P:Wnt receptor signaling pathway; IEA:UniProtKB-KW. 
Interpro
 IPR000938; CAP-Gly_domain.
 IPR018200; Pept_C19ubi-hydrolase_C_CS.
 IPR001394; Peptidase_C19. 
Pfam
 PF01302; CAP_GLY
 PF00443; UCH 
SMART
 SM01052; CAP_GLY 
PROSITE
 PS00845; CAP_GLY_1
 PS50245; CAP_GLY_2
 PS00972; UCH_2_1
 PS00973; UCH_2_2
 PS50235; UCH_2_3 
PRINTS