CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012664
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nuclear factor erythroid 2-related factor 2 
Protein Synonyms/Alias
 NF-E2-related factor 2; NFE2-related factor 2; HEBP1; Nuclear factor, erythroid derived 2, like 2 
Gene Name
 NFE2L2 
Gene Synonyms/Alias
 NRF2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
44FDFSQRRKEYELEKQubiquitination[1]
64ERQEQLQKEQEKAFFubiquitination[1]
438PVSPGHRKTPFTKDKacetylation[2]
443HRKTPFTKDKHSSRLacetylation[2]
445KTPFTKDKHSSRLEAacetylation[2]
462TRDELRAKALHIPFPacetylation[2]
472HIPFPVEKIINLPVVacetylation[2]
506RDIRRRGKNKVAAQNacetylation[2]
508IRRRGKNKVAAQNCRacetylation[2]
518AQNCRKRKLENIVELacetylation[2]
543KEKLLKEKGENDKSLacetylation[2]
548KEKGENDKSLHLLKKacetylation[2]
554DKSLHLLKKQLSTLYacetylation[2]
555KSLHLLKKQLSTLYLacetylation[2]
574MLRDEDGKPYSPSEYubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Acetylation of Nrf2 by p300/CBP augments promoter-specific DNA binding of Nrf2 during the antioxidant response.
 Sun Z, Chin YE, Zhang DD.
 Mol Cell Biol. 2009 May;29(10):2658-72. [PMID: 19273602] 
Functional Description
 Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. 
Sequence Annotation
 DOMAIN 497 560 bZIP.
 REGION 499 518 Basic motif (By similarity).
 REGION 522 529 Leucine-zipper (By similarity).
 MOD_RES 40 40 Phosphoserine; by PKC.
 MOD_RES 215 215 Phosphoserine.
 MOD_RES 596 596 N6-acetyllysine; by CREBBP.
 MOD_RES 599 599 N6-acetyllysine; by CREBBP.  
Keyword
 3D-structure; Acetylation; Activator; Alternative splicing; Complete proteome; Cytoplasm; DNA-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transcription; Transcription regulation; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 605 AA 
Protein Sequence
MMDLELPPPG LPSQQDMDLI DILWRQDIDL GVSREVFDFS QRRKEYELEK QKKLEKERQE 60
QLQKEQEKAF FAQLQLDEET GEFLPIQPAQ HIQSETSGSA NYSQVAHIPK SDALYFDDCM 120
QLLAQTFPFV DDNEVSSATF QSLVPDIPGH IESPVFIATN QAQSPETSVA QVAPVDLDGM 180
QQDIEQVWEE LLSIPELQCL NIENDKLVET TMVPSPEAKL TEVDNYHFYS SIPSMEKEVG 240
NCSPHFLNAF EDSFSSILST EDPNQLTVNS LNSDATVNTD FGDEFYSAFI AEPSISNSMP 300
SPATLSHSLS ELLNGPIDVS DLSLCKAFNQ NHPESTAEFN DSDSGISLNT SPSVASPEHS 360
VESSSYGDTL LGLSDSEVEE LDSAPGSVKQ NGPKTPVHSS GDMVQPLSPS QGQSTHVHDA 420
QCENTPEKEL PVSPGHRKTP FTKDKHSSRL EAHLTRDELR AKALHIPFPV EKIINLPVVD 480
FNEMMSKEQF NEAQLALIRD IRRRGKNKVA AQNCRKRKLE NIVELEQDLD HLKDEKEKLL 540
KEKGENDKSL HLLKKQLSTL YLEVFSMLRD EDGKPYSPSE YSLQQTRDGN VFLVPKSKKP 600
DVKKN 605 
Gene Ontology
 GO:0005813; C:centrosome; IDA:HPA.
 GO:0000785; C:chromatin; IEA:Compara.
 GO:0005829; C:cytosol; IDA:BHF-UCL.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0005886; C:plasma membrane; IDA:HPA.
 GO:0003677; F:DNA binding; IDA:UniProtKB.
 GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; TAS:ProtInc.
 GO:0070301; P:cellular response to hydrogen peroxide; IMP:BHF-UCL.
 GO:0071499; P:cellular response to laminar fluid shear stress; IMP:BHF-UCL.
 GO:0071356; P:cellular response to tumor necrosis factor; IMP:BHF-UCL.
 GO:0030968; P:endoplasmic reticulum unfolded protein response; IEA:Compara.
 GO:0043066; P:negative regulation of apoptotic process; IMP:BHF-UCL.
 GO:0030194; P:positive regulation of blood coagulation; IEA:Compara.
 GO:2000379; P:positive regulation of reactive oxygen species metabolic process; IEA:Compara.
 GO:0036003; P:positive regulation of transcription from RNA polymerase II promoter in response to stress; IMP:BHF-UCL.
 GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; IDA:UniProtKB.
 GO:0010499; P:proteasomal ubiquitin-independent protein catabolic process; IDA:UniProtKB.
 GO:0016567; P:protein ubiquitination; IDA:UniProtKB.
 GO:0045995; P:regulation of embryonic development; IEA:Compara.
 GO:2000121; P:regulation of removal of superoxide radicals; IEA:Compara.
 GO:0006366; P:transcription from RNA polymerase II promoter; TAS:ProtInc. 
Interpro
 IPR004827; bZIP.
 IPR008917; Euk_TF_DNA-bd. 
Pfam
 PF00170; bZIP_1 
SMART
 SM00338; BRLZ 
PROSITE
 PS50217; BZIP
 PS00036; BZIP_BASIC 
PRINTS