CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023084
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 F-box/LRR-repeat protein 4 
Protein Synonyms/Alias
 F-box and leucine-rich repeat protein 4; F-box protein FBL4/FBL5 
Gene Name
 FBXL4 
Gene Synonyms/Alias
 FBL4; FBL5 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
115PSASLPFKRTPPNFQubiquitination[1, 2, 3, 4]
182LWSERPTKVNASQARubiquitination[2]
537HQLPNLQKLFLTANRubiquitination[2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
  
Sequence Annotation
 DOMAIN 277 332 F-box.
 REPEAT 376 397 LRR 1.
 REPEAT 402 421 LRR 2.
 REPEAT 427 448 LRR 3.
 REPEAT 452 474 LRR 4.
 REPEAT 480 501 LRR 5.
 REPEAT 504 524 LRR 6.
 REPEAT 532 558 LRR 7.
 REPEAT 559 583 LRR 8.
 REPEAT 584 609 LRR 9.  
Keyword
 Complete proteome; Cytoplasm; Leucine-rich repeat; Nucleus; Reference proteome; Repeat; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 621 AA 
Protein Sequence
MSPVFPMLTV LTMFYYICLR RRARTATRGE MMNTHRAIES NSQTSPLNAE VVQYAKEVVD 60
FSSHYGSENS MSYTMWNLAG VPNVFPSSGD FTQTAVFRTY GTWWDQCPSA SLPFKRTPPN 120
FQSQDYVELT FEQQVYPTAV HVLETYHPGA VIRILACSAN PYSPNPPAEV RWEILWSERP 180
TKVNASQARQ FKPCIKQINF PTNLIRLEVN SSLLEYYTEL DAVVLHGVKD KPVLSLKTSL 240
IDMNDIEDDA YAEKDGCGMD SLNKKFSSAV LGEGPNNGYF DKLPYELIQL ILNHLTLPDL 300
CRLAQTCKLL SQHCCDPLQY IHLNLQPYWA KLDDTSLEFL QSRCTLVQWL NLSWTGNRGF 360
ISVAGFSRFL KVCGSELVRL ELSCSHFLNE TCLEVISEMC PNLQALNLSS CDKLPPQAFN 420
HIAKLCSLKR LVLYRTKVEQ TALLSILNFC SELQHLSLGS CVMIEDYDVI ASMIGAKCKK 480
LRTLDLWRCK NITENGIAEL ASGCPLLEEL DLGWCPTLQS STGCFTRLAH QLPNLQKLFL 540
TANRSVCDTD IDELACNCTR LQQLDILGTR MVSPASLRKL LESCKDLSLL DVSFCSQIDN 600
RAVLELNASF PKVFIKKSFT Q 621 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0000151; C:ubiquitin ligase complex; TAS:ProtInc.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; TAS:ProtInc. 
Interpro
 IPR001810; F-box_dom_cyclin-like.
 IPR006553; Leu-rich_rpt_Cys-con_subtyp. 
Pfam
 PF00646; F-box 
SMART
 SM00256; FBOX
 SM00367; LRR_CC 
PROSITE
 PS50181; FBOX 
PRINTS