CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002493
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cathepsin D 
Protein Synonyms/Alias
 Cathepsin D light chain; Cathepsin D heavy chain 
Gene Name
 CTSD 
Gene Synonyms/Alias
 CPSD 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
28LVRIPLHKFTSIRRTubiquitination[1, 2]
54IAKGPVSKYSQAVPAubiquitination[1, 2]
184QVFGEATKQPGITFIubiquitination[1, 2, 3]
253MLGGTDSKYYKGSLSubiquitination[4]
313DEVRELQKAIGAVPLubiquitination[3]
331EYMIPCEKVSTLPAIubiquitination[3]
341TLPAITLKLGGKGYKubiquitination[3]
348KLGGKGYKLSPEDYTubiquitination[1, 2, 3]
357SPEDYTLKVSQAGKTubiquitination[3]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. 
Sequence Annotation
 ACT_SITE 97 97
 ACT_SITE 295 295
 CARBOHYD 63 63 O-linked (GalNAc...).
 CARBOHYD 134 134 N-linked (GlcNAc...).
 CARBOHYD 263 263 N-linked (GlcNAc...).
 DISULFID 91 160
 DISULFID 110 117
 DISULFID 286 290
 DISULFID 329 366  
Keyword
 3D-structure; Alzheimer disease; Aspartyl protease; Complete proteome; Direct protein sequencing; Disease mutation; Disulfide bond; Glycoprotein; Hydrolase; Lysosome; Neurodegeneration; Neuronal ceroid lipofuscinosis; Polymorphism; Protease; Reference proteome; Secreted; Signal; Zymogen. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 412 AA 
Protein Sequence
MQPSSLLPLA LCLLAAPASA LVRIPLHKFT SIRRTMSEVG GSVEDLIAKG PVSKYSQAVP 60
AVTEGPIPEV LKNYMDAQYY GEIGIGTPPQ CFTVVFDTGS SNLWVPSIHC KLLDIACWIH 120
HKYNSDKSST YVKNGTSFDI HYGSGSLSGY LSQDTVSVPC QSASSASALG GVKVERQVFG 180
EATKQPGITF IAAKFDGILG MAYPRISVNN VLPVFDNLMQ QKLVDQNIFS FYLSRDPDAQ 240
PGGELMLGGT DSKYYKGSLS YLNVTRKAYW QVHLDQVEVA SGLTLCKEGC EAIVDTGTSL 300
MVGPVDEVRE LQKAIGAVPL IQGEYMIPCE KVSTLPAITL KLGGKGYKLS PEDYTLKVSQ 360
AGKTLCLSGF MGMDIPPPSG PLWILGDVFI GRYYTVFDRD NNRVGFAEAA RL 412 
Gene Ontology
 GO:0005615; C:extracellular space; IDA:BHF-UCL.
 GO:0043202; C:lysosomal lumen; TAS:Reactome.
 GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0004190; F:aspartic-type endopeptidase activity; TAS:ProtInc.
 GO:0019886; P:antigen processing and presentation of exogenous peptide antigen via MHC class II; TAS:Reactome.
 GO:0000045; P:autophagic vacuole assembly; IEA:Compara.
 GO:0008219; P:cell death; IEA:UniProtKB-KW.
 GO:0030574; P:collagen catabolic process; TAS:Reactome.
 GO:0022617; P:extracellular matrix disassembly; TAS:Reactome.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW. 
Interpro
 IPR001461; Peptidase_A1.
 IPR021109; Peptidase_aspartic.
 IPR001969; Peptidase_aspartic_AS.
 IPR012848; Propep_A1. 
Pfam
 PF07966; A1_Propeptide
 PF00026; Asp 
SMART
  
PROSITE
 PS00141; ASP_PROTEASE 
PRINTS
 PR00792; PEPSIN.