CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-040914
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 E3 ubiquitin-protein ligase RNF149 
Protein Synonyms/Alias
  
Gene Name
 RNF149 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
245SHRKETKKVIGQLLLubiquitination[1, 2]
260HTVKHGEKGIDVDAEubiquitination[2, 3]
279CIENFKVKDIIRILPubiquitination[2]
288IIRILPCKHIFHRICubiquitination[2, 4]
310HRTCPMCKLDVIKALubiquitination[2, 3, 4]
315MCKLDVIKALGYWGEubiquitination[5]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Metal-binding; Reference proteome; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 398 AA 
Protein Sequence
MAWRRREASV GARGVLALAL LALALCVPGA RGRALEWFSA VVNIEYVDPQ TNLTVWSVSE 60
SGRFGDSSPK EGAHGLVGVP WAPGGDLEGC APDTRFFVPE PGGRGAAPWV ALVARGGCTF 120
KDKVLVAARR NASAVVLYNE ERYGNITLPM SHAGTGNIVV IMISYPKGRE ILELVQKGIP 180
VTMTIGVGTR HVQEFISGQS VVFVAIAFIT MMIISLAWLI FYYIQRFLYT GSQIGSQSHR 240
KETKKVIGQL LLHTVKHGEK GIDVDAENCA VCIENFKVKD IIRILPCKHI FHRICIDPWL 300
LDHRTCPMCK LDVIKALGYW GEPGDVQEMP APESPPGRDP AANLSLALPD DDGSDDSSPP 360
SASPAESEPQ CDPSFKGDAG ENTALLGAMC SHVMWTSI 398 
Gene Ontology
 GO:0008270; F:zinc ion binding; IEA:InterPro. 
Interpro
 IPR003137; Protease-assoc_domain.
 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD. 
Pfam
 PF02225; PA
 PF13639; zf-RING_2 
SMART
 SM00184; RING 
PROSITE
 PS50089; ZF_RING_2 
PRINTS