CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021423
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Pecanex-like protein 3 
Protein Synonyms/Alias
  
Gene Name
 PCNXL3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
762PRAQHSYKYWLLPGRubiquitination[1, 2, 3]
1016LWSLIRSKLFPELEEubiquitination[4, 5]
1040PPDPLPDKMRQSVREubiquitination[1, 3, 6]
1103YLLPQLRKQLPWFCLubiquitination[2]
1116CLSQPVLKPLEYSQYubiquitination[2]
1187LMTVAGLKLLRSAFCubiquitination[6]
1307MSYARPLKFWERDYNubiquitination[1, 3]
1762RGSIQNAKQALRNMIubiquitination[1, 3, 4, 7, 8]
1935SSEGPSGKWSLGGRKubiquitination[1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [7] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [8] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [9] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [10] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [11] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661
Functional Description
  
Sequence Annotation
 MOD_RES 127 127 Phosphoserine (By similarity).
 MOD_RES 370 370 Phosphothreonine.
 MOD_RES 505 505 Phosphoserine (By similarity).
 CARBOHYD 319 319 N-linked (GlcNAc...) (Potential).
 CARBOHYD 1770 1770 N-linked (GlcNAc...) (Potential).  
Keyword
 Alternative splicing; Complete proteome; Glycoprotein; Membrane; Phosphoprotein; Polymorphism; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2034 AA 
Protein Sequence
MGSQVLQILR QGVWASLTGG WFFDPHQSTF SNCFHLYVWI FLLIFPFLLY MVLPPSLMVA 60
GVYCLVVAVI FATIKTVNYR LHAMFDQGEI VEKRSSTMGE LEEEPAQGDS NPPRDPGVEM 120
TVFRKVSSTP PVRCSSQHSV FGFNQVSELL PRMEDSGPLR DIKELVREQG SNNVIVTSAD 180
REMLKLSSQE KLIGDLPQTP PGAVPDPSLA STDSSEPSPL AGDGAPWSGS SMADTPMSPL 240
LKGSLSQELS KSFLTLTQPD RALVRTSSRR EQRRGAGGYQ PLDRRGSGEP TPQKAGSSDS 300
CFSGTDRETL SSFKSEKTNS THLDSPPGGP APEGSDTDPP SEAELPASPD AGVPSDDTLR 360
SFDTVIGAGT PPGLAEPLLV VRPKDLALLR PSKRQPPLRR HSPPGRAPRR PLLEGGGFFE 420
DEDTSEGSEL SPASSLRSQR RYSTDSSSST SCYSPESSRG AAGGPRKRRA PHGAEEGTAV 480
PPKRPYGTQR TPSTASAKTH ARVLSMDGAG GDVLRPPLAG CKAELEAQVG VEQAASEPVV 540
LPAEARRGPA ANQPGWRGEL QEEGAVGGAA EETGRRDRSS SVRRTQAIRR RHNAGSNPTP 600
PASVMGSPPS SLQEAQRGRA ASHSRALTLP SALHFASSLL LTRAGANVHE ACTFDDTSEG 660
AVHYFYDESG VRRSYTFGLA GGGYENPVGQ QGEQTANGAW DRHSHSSSFH SADVPEATGG 720
LNLLQPRPVV LQGMQVRRVP LEIPEEQTLM EEAPPRAQHS YKYWLLPGRW TSVRYERLAL 780
LALLDRTRGV LENIFGVGLS SLVAFLGYLL LLKGFFTDIW VFQFCLVIAS CQYSLLKSVQ 840
PDAASPMHGH NWVIAYSRPV YFCICCLLIW LLDALGSAQP FPPVSLYGLT LFSASFFFCA 900
RDVATVFTLC FPFVFLLGLL PQVNTCLMYL LEQIDMHGFG GTAATSPLTA VFSLSRSLLA 960
AALLYGFCLG AIKTPWPEQH VPVLFSVFCG LLVALSYHLS RQSSDPTVLW SLIRSKLFPE 1020
LEERSLETAR AEPPDPLPDK MRQSVREVLH SDLVMCVVIA VLTFAISAST VFIALKSVLG 1080
FVLYALAGAV GFFTHYLLPQ LRKQLPWFCL SQPVLKPLEY SQYEVRGAAQ VMWFEKLYAG 1140
LQCVEKYLIY PAVVLNALTV DAHTVVSHPD KYCFYCRALL MTVAGLKLLR SAFCCPPQQY 1200
LTLAFTVLLF HFDYPRLSQG FLLDYFLMSL LCSKLWDLLY KLRFVLTYIA PWQITWGSAF 1260
HAFAQPFAVP HSAMLFVQAL LSGLFSTPLN PLLGSAVFIM SYARPLKFWE RDYNTKRVDH 1320
SNTRLVTQLD RNPGADDNNL NSIFYEHLTR SLQHTLCGDL VLGRWGNYGP GDCFVLASDY 1380
LNALVHLIEV GNGLVTFQLR GLEFRGTYCQ QREVEAITEG VEEDEGCCCC EPGHLPRVLS 1440
FNAAFGQRWL AWEVTASKYV LEGYSISDNN AASMLQVFDL RKILITYYVK SIIYYVSRSP 1500
KLEVWLSHEG ITAALRPVRV PGYADSDPTF SLSVDEDYDL RLSGLSLPSF CAVHLEWIQY 1560
CASRRSQPVD QDWNSPLVTL CFGLCVLGRR ALGTASHSMS ASLEPFLYGL HALFKGDFRI 1620
TSPRDEWVFA DMDLLHRVVA PGVRMALKLH QDHFTSPDEY EEPAALYDAI AANEERLVIS 1680
HEGDPAWRSA ILSNTPSLLA LRHVLDDASD EYKIIMLNRR HLSFRVIKVN RECVRGLWAG 1740
QQQELVFLRN RNPERGSIQN AKQALRNMIN SSCDQPLGYP IYVSPLTTSL AGSHPQLRAL 1800
WGGPISLGAI AHWLLRTWER LHKGCGAGCN SGGNVDDSDC SGGGGLTSLS NNPPVAHPTP 1860
ENTAGNGDQP LPPGPGWGPR SSLSGSGDGR PPPLLQWPPP RLPGPPPASP IPTEGPRTSR 1920
PPGPGLLSSE GPSGKWSLGG RKGLGGSDGE PASGSPKGGT PKSQAPLDLS LSLSLSLSPD 1980
VSTEASPPRA SQDIPCLDSS APESGTPMGA LGDWPAPIEE RESPAAQPLL EHQY 2034 
Gene Ontology
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW. 
Interpro
 IPR007735; Pecanex. 
Pfam
 PF05041; Pecanex_C 
SMART
  
PROSITE
  
PRINTS