UniProt Accession

 TCPH_MOUSE; P80313 

Genbank Protein ID

 Z31399; AB022160; BC008255 

Genbank Nucleotide ID

 CAA83274.1; BAA81878.1; AAH08255.1 

Protein Name

 T-complex protein 1 subunit eta 

Protein Synonyms/Alias

 TCP-1-eta; CCT-eta 

Gene Name

 Cct7 

Gene Synonyms/Alias

 Ccth 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
47	LGPRGMDKLIVDGRG	ubiquitination	[1]
55	LIVDGRGKATISNDG	acetylation	[2]
55	LIVDGRGKATISNDG	succinylation	[2]
55	LIVDGRGKATISNDG	ubiquitination	[1]
67	NDGATILKLLDVVHP	acetylation	[3]
67	NDGATILKLLDVVHP	ubiquitination	[1]
77	DVVHPAAKTLVDIAK	ubiquitination	[1]
109	AEFLKQVKPYVEEGL	acetylation	[2]
157	EQRKMLEKCAMTALS	acetylation	[3]
172	SKLISQQKVFFAKMV	acetylation	[2, 3, 4]
172	SKLISQQKVFFAKMV	succinylation	[2]
218	VAGVAFKKTFSYAGF	acetylation	[3]
218	VAGVAFKKTFSYAGF	ubiquitination	[1]
230	AGFEMQPKKYKNPKI	ubiquitination	[1]
247	LNVELELKAEKDNAE	ubiquitination	[1]
250	ELELKAEKDNAEIRV	acetylation	[2]
292	GAKVILSKLPIGDVA	acetylation	[3]
366	NFFTGCPKAKTCTII	acetylation	[2]
366	NFFTGCPKAKTCTII	succinylation	[2]
418	AIEMELSKYLRDYSR	ubiquitination	[1]
430	YSRTIPGKQQLLIGA	ubiquitination	[1]
463	DATNILNKLRARHAQ	acetylation	[5]
463	DATNILNKLRARHAQ	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [3] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [5] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654] 

Functional Description

 Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin. 

Sequence Annotation

 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 67 67 N6-acetyllysine (By similarity).
 MOD_RES 320 320 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; ATP-binding; Chaperone; Complete proteome; Cytoplasm; Direct protein sequencing; Nucleotide-binding; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 544 AA 

Protein Sequence

Gene Ontology

 GO:0044297; C:cell body; IDA:MGI.
 GO:0005832; C:chaperonin-containing T-complex; IDA:MGI.
 GO:0005874; C:microtubule; IEA:Compara.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0002199; C:zona pellucida receptor complex; IDA:MGI.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0007339; P:binding of sperm to zona pellucida; IDA:MGI.
 GO:0006457; P:protein folding; IEA:InterPro. 

Interpro

 IPR012720; Chap_CCT_eta.
 IPR017998; Chaperone_TCP-1.
 IPR002194; Chaperonin_TCP-1_CS.
 IPR002423; Cpn60/TCP-1.
 IPR027409; GroEL-like_apical_dom.
 IPR027413; GROEL-like_equatorial.
 IPR027410; TCP-1-like_intermed. 

Pfam

 PF00118; Cpn60_TCP1 

SMART

  

PROSITE

 PS00750; TCP1_1
 PS00751; TCP1_2
 PS00995; TCP1_3 

PRINTS

 PR00304; TCOMPLEXTCP1.