UniProt Accession

 FABH_ECOLI; P0A6R0; P24249 

Genbank Protein ID

 M77744; M96793; U00096; AP009048; M87040; Z11565 

Genbank Nucleotide ID

 AAA23749.1; AAB59065.1; AAC74175.1; BAA35899.2; AAA23741.1; CAA77659.1 

Protein Name

 3-oxoacyl-[acyl-carrier-protein] synthase 3 

Protein Synonyms/Alias

 3-oxoacyl-[acyl-carrier-protein] synthase III; Beta-ketoacyl-ACP synthase III; KAS III; EcFabH 

Gene Name

 fabH 

Gene Synonyms/Alias

 b1091; JW1077 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
24	RTNADLEKMVDTSDE	acetylation	[1]
132	YVKSGAVKYALVVGS	acetylation	[1]
257	IISATAKKLGMSMDN	acetylation	[1, 2]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 

Functional Description

 Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Has some substrate specificity for acetyl-CoA. Its substrate specificity determines the biosynthesis of straight-chain of fatty acids instead of branched-chain. 

Sequence Annotation

 REGION 245 249 ACP-binding.
 ACT_SITE 112 112
 ACT_SITE 244 244
 ACT_SITE 274 274  

Keyword

 3D-structure; Acyltransferase; Complete proteome; Cytoplasm; Direct protein sequencing; Fatty acid biosynthesis; Fatty acid metabolism; Lipid biosynthesis; Lipid metabolism; Multifunctional enzyme; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 317 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
 GO:0033818; F:beta-ketoacyl-acyl-carrier-protein synthase III activity; IDA:EcoCyc.
 GO:0006633; P:fatty acid biosynthetic process; IEA:HAMAP. 

Interpro

 IPR013751; ACP_syn_III.
 IPR013747; ACP_syn_III_C.
 IPR004655; FabH_synth.
 IPR016039; Thiolase-like.
 IPR016038; Thiolase-like_subgr. 

Pfam

 PF08545; ACP_syn_III
 PF08541; ACP_syn_III_C 

SMART

  

PROSITE

  

PRINTS