UniProt Accession

 ILV5_YEAST; P06168; D6VYZ3 

Genbank Protein ID

 X04969; U19102; BK006945 

Genbank Nucleotide ID

 CAA28643.1; AAB67753.1; DAA09659.1 

Protein Name

 Ketol-acid reductoisomerase, mitochondrial 

Protein Synonyms/Alias

 Acetohydroxy-acid reductoisomerase; Alpha-keto-beta-hydroxylacyl reductoisomerase 

Gene Name

 ILV5 

Gene Synonyms/Alias

 YLR355C; L9638.7 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
70	RADWPREKLLDYFKN	acetylation	[1]
115	RKDGASWKAAIEDGW	acetylation	[1]
126	EDGWVPGKNLFTVED	acetylation	[1]
136	FTVEDAIKRGSYVMN	acetylation	[1]
165	KPLLTKGKTLYFSHG	acetylation	[1]
178	HGFSPVFKDLTHVEP	acetylation	[1]
187	LTHVEPPKDLDVILV	acetylation	[1]
208	RTVRSLFKEGRGINS	acetylation	[1]
226	VWNDVTGKAHEKAQA	acetylation	[1]
336	PIFKNALKPVFQDLY	acetylation	[1]
347	QDLYESTKNGTETKR	acetylation	[1]
367	SQPDYREKLEKELDT	acetylation	[1]
370	DYREKLEKELDTIRN	acetylation	[1]
382	IRNMEIWKVGKEVRK	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

  

Sequence Annotation

 NP_BIND 84 93 NADP (Potential).
 NP_BIND 108 113 NADP (By similarity).
 NP_BIND 146 150 NADP (By similarity).
 REGION 363 395 Hydrophilic.
 ACT_SITE 171 171 Potential.
 METAL 255 255 Magnesium 1 (By similarity).
 METAL 255 255 Magnesium 2 (By similarity).
 METAL 259 259 Magnesium 1 (By similarity).
 MOD_RES 355 355 Phosphoserine.  

Keyword

 Amino-acid biosynthesis; Branched-chain amino acid biosynthesis; Complete proteome; Direct protein sequencing; Magnesium; Metal-binding; Mitochondrion; NADP; Oxidoreductase; Phosphoprotein; Reference proteome; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 395 AA 

Protein Sequence

Gene Ontology

 GO:0042645; C:mitochondrial nucleoid; IDA:SGD.
 GO:0050662; F:coenzyme binding; IEA:InterPro.
 GO:0003690; F:double-stranded DNA binding; IDA:SGD.
 GO:0004455; F:ketol-acid reductoisomerase activity; IMP:SGD.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0009082; P:branched-chain amino acid biosynthetic process; IMP:SGD.
 GO:0009097; P:isoleucine biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0000002; P:mitochondrial genome maintenance; IMP:SGD.
 GO:0009099; P:valine biosynthetic process; IEA:UniProtKB-UniPathway. 

Interpro

 IPR008927; 6-PGluconate_DH_C-like.
 IPR013023; AcH_isomrdctse.
 IPR000506; AcH_isomrdctse_C.
 IPR013328; DH_multihelical.
 IPR013116; IlvN.
 IPR016207; KetolA_reductoisomerase_fun. 

Pfam

 PF01450; IlvC
 PF07991; IlvN 

SMART

  

PROSITE

  

PRINTS