CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004698
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Regulator of chromosome condensation 
Protein Synonyms/Alias
 Cell cycle regulatory protein; Chromosome condensation protein 1 
Gene Name
 RCC1 
Gene Synonyms/Alias
 CHC1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
114GSEMVPGKVELQEKVubiquitination[1]
160IGLLEPMKKSMVPVQubiquitination[1]
161GLLEPMKKSMVPVQVubiquitination[2]
335LGEGAEEKSIPTLISubiquitination[1]
362SVGYAVTKDGRVFAWubiquitination[3, 4, 5]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo- cytoplasmic transport, mitosis and nuclear-envelope assembly. 
Sequence Annotation
 REPEAT 34 84 RCC1 1.
 REPEAT 85 136 RCC1 2.
 REPEAT 138 189 RCC1 3.
 REPEAT 191 257 RCC1 4.
 REPEAT 258 311 RCC1 5.
 REPEAT 312 362 RCC1 6.
 REPEAT 363 416 RCC1 7.
 MOD_RES 2 2 N,N,N-trimethylserine; alternate.
 MOD_RES 2 2 N,N-dimethylserine; alternate.
 MOD_RES 2 2 N-methylserine; alternate.
 MOD_RES 2 2 Phosphoserine.
 MOD_RES 11 11 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; Cell cycle; Cell division; Complete proteome; Cytoplasm; Direct protein sequencing; DNA-binding; Guanine-nucleotide releasing factor; Methylation; Mitosis; Nucleus; Phosphoprotein; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 421 AA 
Protein Sequence
MSPKRIAKRR SPPADAIPKS KKVKVSHRSH STEPGLVLTL GQGDVGQLGL GENVMERKKP 60
ALVSIPEDVV QAEAGGMHTV CLSKSGQVYS FGCNDEGALG RDTSVEGSEM VPGKVELQEK 120
VVQVSAGDSH TAALTDDGRV FLWGSFRDNN GVIGLLEPMK KSMVPVQVQL DVPVVKVASG 180
NDHLVMLTAD GDLYTLGCGE QGQLGRVPEL FANRGGRQGL ERLLVPKCVM LKSRGSRGHV 240
RFQDAFCGAY FTFAISHEGH VYGFGLSNYH QLGTPGTESC FIPQNLTSFK NSTKSWVGFS 300
GGQHHTVCMD SEGKAYSLGR AEYGRLGLGE GAEEKSIPTL ISRLPAVSSV ACGASVGYAV 360
TKDGRVFAWG MGTNYQLGTG QDEDAWSPVE MMGKQLENRV VLSVSSGGQH TVLLVKDKEQ 420
S 421 
Gene Ontology
 GO:0000794; C:condensed nuclear chromosome; IDA:UniProtKB.
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0000790; C:nuclear chromatin; IDA:UniProtKB.
 GO:0031965; C:nuclear membrane; IDA:HPA.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0042393; F:histone binding; IDA:UniProtKB.
 GO:0031492; F:nucleosomal DNA binding; IDA:UniProtKB.
 GO:0005087; F:Ran guanyl-nucleotide exchange factor activity; IDA:UniProtKB.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007059; P:chromosome segregation; IMP:UniProtKB.
 GO:0000082; P:G1/S transition of mitotic cell cycle; IMP:UniProtKB.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0007052; P:mitotic spindle organization; IDA:UniProtKB.
 GO:0007088; P:regulation of mitosis; IDA:UniProtKB.
 GO:0051225; P:spindle assembly; IMP:UniProtKB.
 GO:0022415; P:viral reproductive process; TAS:Reactome. 
Interpro
 IPR009091; RCC1/BLIP-II.
 IPR000408; Reg_chr_condens. 
Pfam
 PF00415; RCC1 
SMART
  
PROSITE
 PS00625; RCC1_1
 PS00626; RCC1_2
 PS50012; RCC1_3 
PRINTS
 PR00633; RCCNDNSATION.