CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000455
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Canalicular multispecific organic anion transporter 2 
Protein Synonyms/Alias
 ATP-binding cassette sub-family C member 3; Multi-specific organic anion transporter D; MOAT-D; Multidrug resistance-associated protein 3 
Gene Name
 ABCC3 
Gene Synonyms/Alias
 CMOAT2; MLP2; MRP3 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
244EKDLWSLKEEDRSQMubiquitination[1, 2]
278KASAAPGKNASGEDEubiquitination[2]
392IMGVIYRKALVITNSubiquitination[1]
401LVITNSVKRASTVGEubiquitination[1]
499SEILNGIKVLKLYAWubiquitination[2]
512AWEPSFLKQVEGIRQubiquitination[1, 2, 3, 4]
803PEGVLAGKTRVLVTHubiquitination[2]
931RHLGPSEKVQVTEAKubiquitination[1, 2]
938KVQVTEAKADGALTQubiquitination[1, 2, 4]
1177FEIISDTKVDANQRSubiquitination[1, 2]
1344FRILEAAKGEIRIDGubiquitination[1]
1498TRVLVLDKGVVAEFDubiquitination[1, 2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572] 
Functional Description
 May act as an inducible transporter in the biliary and intestinal excretion of organic anions. Acts as an alternative route for the export of bile acids and glucuronides from cholestatic hepatocytes (By similarity). 
Sequence Annotation
 DOMAIN 311 594 ABC transmembrane type-1 1.
 DOMAIN 629 851 ABC transporter 1.
 DOMAIN 971 1252 ABC transmembrane type-1 2.
 DOMAIN 1291 1523 ABC transporter 2.
 NP_BIND 661 668 ATP 1 (Potential).
 NP_BIND 1323 1330 ATP 2 (Potential).
 MOD_RES 908 908 Phosphoserine (By similarity).
 CARBOHYD 18 18 N-linked (GlcNAc...) (Potential).
 CARBOHYD 1006 1006 N-linked (GlcNAc...) (Potential).
 CARBOHYD 1007 1007 N-linked (GlcNAc...) (Potential).  
Keyword
 Alternative splicing; ATP-binding; Complete proteome; Glycoprotein; Membrane; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1527 AA 
Protein Sequence
MDALCGSGEL GSKFWDSNLS VHTENPDLTP CFQNSLLAWV PCIYLWVALP CYLLYLRHHC 60
RGYIILSHLS KLKMVLGVLL WCVSWADLFY SFHGLVHGRA PAPVFFVTPL VVGVTMLLAT 120
LLIQYERLQG VQSSGVLIIF WFLCVVCAIV PFRSKILLAK AEGEISDPFR FTTFYIHFAL 180
VLSALILACF REKPPFFSAK NVDPNPYPET SAGFLSRLFF WWFTKMAIYG YRHPLEEKDL 240
WSLKEEDRSQ MVVQQLLEAW RKQEKQTARH KASAAPGKNA SGEDEVLLGA RPRPRKPSFL 300
KALLATFGSS FLISACFKLI QDLLSFINPQ LLSILIRFIS NPMAPSWWGF LVAGLMFLCS 360
MMQSLILQHY YHYIFVTGVK FRTGIMGVIY RKALVITNSV KRASTVGEIV NLMSVDAQRF 420
MDLAPFLNLL WSAPLQIILA IYFLWQNLGP SVLAGVAFMV LLIPLNGAVA VKMRAFQVKQ 480
MKLKDSRIKL MSEILNGIKV LKLYAWEPSF LKQVEGIRQG ELQLLRTAAY LHTTTTFTWM 540
CSPFLVTLIT LWVYVYVDPN NVLDAEKAFV SVSLFNILRL PLNMLPQLIS NLTQASVSLK 600
RIQQFLSQEE LDPQSVERKT ISPGYAITIH SGTFTWAQDL PPTLHSLDIQ VPKGALVAVV 660
GPVGCGKSSL VSALLGEMEK LEGKVHMKGS VAYVPQQAWI QNCTLQENVL FGKALNPKRY 720
QQTLEACALL ADLEMLPGGD QTEIGEKGIN LSGGQRQRVS LARAVYSDAD IFLLDDPLSA 780
VDSHVAKHIF DHVIGPEGVL AGKTRVLVTH GISFLPQTDF IIVLADGQVS EMGPYPALLQ 840
RNGSFANFLC NYAPDEDQGH LEDSWTALEG AEDKEALLIE DTLSNHTDLT DNDPVTYVVQ 900
KQFMRQLSAL SSDGEGQGRP VPRRHLGPSE KVQVTEAKAD GALTQEEKAA IGTVELSVFW 960
DYAKAVGLCT TLAICLLYVG QSAAAIGANV WLSAWTNDAM ADSRQNNTSL RLGVYAALGI 1020
LQGFLVMLAA MAMAAGGIQA ARVLHQALLH NKIRSPQSFF DTTPSGRILN CFSKDIYVVD 1080
EVLAPVILML LNSFFNAIST LVVIMASTPL FTVVILPLAV LYTLVQRFYA ATSRQLKRLE 1140
SVSRSPIYSH FSETVTGASV IRAYNRSRDF EIISDTKVDA NQRSCYPYII SNRWLSIGVE 1200
FVGNCVVLFA ALFAVIGRSS LNPGLVGLSV SYSLQVTFAL NWMIRMMSDL ESNIVAVERV 1260
KEYSKTETEA PWVVEGSRPP EGWPPRGEVE FRNYSVRYRP GLDLVLRDLS LHVHGGEKVG 1320
IVGRTGAGKS SMTLCLFRIL EAAKGEIRID GLNVADIGLH DLRSQLTIIP QDPILFSGTL 1380
RMNLDPFGSY SEEDIWWALE LSHLHTFVSS QPAGLDFQCS EGGENLSVGQ RQLVCLARAL 1440
LRKSRILVLD EATAAIDLET DNLIQATIRT QFDTCTVLTI AHRLNTIMDY TRVLVLDKGV 1500
VAEFDSPANL IAARGIFYGM ARDAGLA 1527 
Gene Ontology
 GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
 GO:0005524; F:ATP binding; TAS:ProtInc.
 GO:0042626; F:ATPase activity, coupled to transmembrane movement of substances; TAS:ProtInc.
 GO:0008514; F:organic anion transmembrane transporter activity; TAS:ProtInc.
 GO:0015721; P:bile acid and bile salt transport; TAS:Reactome.
 GO:0008206; P:bile acid metabolic process; TAS:Reactome. 
Interpro
 IPR003593; AAA+_ATPase.
 IPR003439; ABC_transporter-like.
 IPR017871; ABC_transporter_CS.
 IPR017940; ABC_transporter_type1.
 IPR001140; ABC_transptr_TM_dom.
 IPR011527; ABC_transptrTM_dom_typ1.
 IPR005292; Multidrug-R_assoc.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00664; ABC_membrane
 PF00005; ABC_tran 
SMART
 SM00382; AAA 
PROSITE
 PS50929; ABC_TM1F
 PS00211; ABC_TRANSPORTER_1
 PS50893; ABC_TRANSPORTER_2 
PRINTS