CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-028318
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 3-isopropylmalate dehydrogenase 
Protein Synonyms/Alias
 3-IPM-DH; Beta-IPM dehydrogenase 
Gene Name
 leuB 
Gene Synonyms/Alias
 MSMEG_2379; MSMEI_2319 
Created Date
 July 27, 2013 
Organism
 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) 
NCBI Taxa ID
 246196 
Lysine Modification
Position
Peptide
Type
References
313EAAARVDKAVSEHLApupylation[1]
Reference
 [1] Proteome-wide identification of mycobacterial pupylation targets.
 Poulsen C, Akhter Y, Jeon AH, Schmitt-Ulms G, Meyer HE, Stefanski A, Stühler K, Wilmanns M, Song YH.
 Mol Syst Biol. 2010 Jul 13;6:386. [PMID: 20631680] 
Functional Description
 Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate (By similarity). 
Sequence Annotation
 NP_BIND 275 287 NAD (By similarity).
 METAL 215 215 Magnesium or manganese (By similarity).
 METAL 239 239 Magnesium or manganese (By similarity).
 METAL 243 243 Magnesium or manganese (By similarity).
 BINDING 91 91 Substrate (By similarity).
 BINDING 101 101 Substrate (By similarity).
 BINDING 125 125 Substrate (By similarity).
 BINDING 215 215 Substrate (By similarity).  
Keyword
 Amino-acid biosynthesis; Branched-chain amino acid biosynthesis; Complete proteome; Cytoplasm; Leucine biosynthesis; Magnesium; Manganese; Metal-binding; NAD; Oxidoreductase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 340 AA 
Protein Sequence
MSSNLKLAVI AGDGIGPEVI AEAVTVLDAV LPGVEKTEYD LGARRYHATG ELLSDETIEE 60
LRGYDAILLG AIGDPSVPSG VLERGMLLKL RFALDHHVNL RPGRLYPGVT GPLVGNPPID 120
FVVVREGTEG PYTGNGGALR VGTPHEVATE VSVNTAFGVE RVVRDAFARA QDRRKHLTLV 180
HKTNVLAFAG SLWSRTVDRV AQEYPDVEVA YQHIDAATIH MVTDPGRFDV IVTDNLFGDI 240
ITDLAAAVCG GIGLAASGNI DATRTNPSMF EPVHGSAPDI AGQGIADPTA AVMSVGLLLS 300
HVGQTEAAAR VDKAVSEHLA TRGDEKLSTS AVGERIRSLL 340 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0003862; F:3-isopropylmalate dehydrogenase activity; IEA:HAMAP.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0051287; F:NAD binding; IEA:InterPro.
 GO:0009098; P:leucine biosynthetic process; IEA:HAMAP. 
Interpro
 IPR023698; 3-isopropylmalate_DH_LeuB.
 IPR019818; IsoCit/isopropylmalate_DH_CS.
 IPR001804; Isocitrate/isopropylmalate_DH.
 IPR024084; IsoPropMal-DH-like_dom. 
Pfam
 PF00180; Iso_dh 
SMART
  
PROSITE
 PS00470; IDH_IMDH 
PRINTS