CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001600
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 GPI ethanolamine phosphate transferase 1 
Protein Synonyms/Alias
 MCD4 homolog; Phosphatidylinositol-glycan biosynthesis class N protein; PIG-N 
Gene Name
 PIGN 
Gene Synonyms/Alias
 MCD4 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
307QFDDAFLKEWRLENWubiquitination[1]
379KVKMTQKKEVTLPFLubiquitination[1]
391PFLFTPFKLLSDSKQubiquitination[1]
397FKLLSDSKQFNILRKubiquitination[1, 2]
927LRLCGKPKSHFM***ubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 
Functional Description
 Ethanolamine phosphate transferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers ethanolamine phosphate to the first alpha-1,4-linked mannose of the glycosylphosphatidylinositol precursor of GPI-anchor (By similarity). 
Sequence Annotation
 CARBOHYD 128 128 N-linked (GlcNAc...) (Potential).
 CARBOHYD 192 192 N-linked (GlcNAc...) (Potential).
 CARBOHYD 350 350 N-linked (GlcNAc...) (Potential).  
Keyword
 Complete proteome; Disease mutation; Endoplasmic reticulum; Glycoprotein; GPI-anchor biosynthesis; Membrane; Polymorphism; Reference proteome; Transferase; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 931 AA 
Protein Sequence
MLLFFTLGLL IHFVFFASIF DIYFTSPLVH GMTPQFTPLP PPARRLVLFV ADGLRADALY 60
ELDENGNSRA PFIRNIIMHE GSWGISHTRV PTESRPGHVA LIAGFYEDVS AVAKGWKENP 120
VEFDSLFNES KYTWSWGSPD ILPMFAKGAS GDHVYTYSYD AKREDFGAQD ATKLDTWVFD 180
NVKDFFHHAR NNQSLFSKIN EEKIVFFLHL LGIDTNGHAH RPSSRDYKHN IKKVDDGVKE 240
IVSMFNHFYG NDGKTTFIFT SDHGMTDWGS HGAGHPSETL TPLVTWGAGI KYPQRVSAQQ 300
FDDAFLKEWR LENWKRLDVN QADIAPLMTS LIGVPFPLNS VGILPVDYLN NTDLFKAESM 360
FTNAVQILEQ FKVKMTQKKE VTLPFLFTPF KLLSDSKQFN ILRKARSYIK HRKFDEVVSL 420
CKELIHLALK GLSYYHTYDR FFLGVNVVIG FVGWISYASL LIIKSHSNLI KGVSKEVKKP 480
SHLLPCSFVA IGILVAFFLL IQACPWTYYV YGLLPLPIWY AVLREFQVIQ DLVVSVLTYP 540
LSHFVGYLLA FTLGIEVLVL SFFYRYMLTA GLTAFAAWPF LTRLWTRAKM TSLSWTFFSL 600
LLAVFPLMPV VGRKPDISLV MGAGLLVLLL SLCVVTSLMK RKDSFIKEEL LVHLLQVLST 660
VLSMYVVYST QSSLLRKQGL PLMNQIISWA TLASSLVVPL LSSPVLFQRL FSILLSLMST 720
YLLLSTGYEA LFPLVLSCLM FVWINIEQET LQQSGVCCKQ KLTSIQFSYN TDITQFRQLY 780
LDDIRRAFFL VFFLVTAFFG TGNIASINSF DLASVYCFLT VFSPFMMGAL MMWKILIPFV 840
LVMCAFEAVQ LTTQLSSKSL FLIVLVISDI MALHFFFLVK DYGSWLDIGT SISHYVIVMS 900
MTIFLVFLNG LAQLLTTKKL RLCGKPKSHF M 931 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; TAS:Reactome.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
 GO:0006501; P:C-terminal protein lipidation; TAS:Reactome.
 GO:0016254; P:preassembly of GPI anchor in ER membrane; TAS:Reactome. 
Interpro
 IPR017849; Alkaline_Pase-like_a/b/a.
 IPR017850; Alkaline_phosphatase_core.
 IPR007070; GPI_EtnP_transferase_1.
 IPR017852; GPI_EtnP_transferase_1_C.
 IPR002591; Phosphodiest/P_Trfase. 
Pfam
 PF01663; Phosphodiest
 PF04987; PigN 
SMART
  
PROSITE
  
PRINTS