UniProt Accession

 CSN5_MOUSE; O35864; Q3UA70; Q8C1S1 

Genbank Protein ID

 U70736; AF068223; AF065386; AK012499; AK146271; AK151492; BC046753 

Genbank Nucleotide ID

 AAD03470.1; AAF61318.1; AAC17179.1; BAB28282.1; BAE27030.1; BAE30445.1; AAH46753.1 

Protein Name

 COP9 signalosome complex subunit 5 

Protein Synonyms/Alias

 SGN5; Signalosome subunit 5; Jun activation domain-binding protein 1; Kip1 C-terminus-interacting protein 2 

Gene Name

 Cops5 

Gene Synonyms/Alias

 Csn5; Jab1; Kic2 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
56	HHYFKYCKISALALL	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. Promotes the proteasomal degradation of BRSK2 (By similarity). The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. Interacts directly with a large number of proteins that are regulated by the CSN complex, confirming a key role in the complex. 

Sequence Annotation

 DOMAIN 53 164 MPN.
 MOTIF 138 151 JAMM motif.
 METAL 138 138 Zinc; catalytic (By similarity).
 METAL 140 140 Zinc; catalytic (By similarity).
 METAL 151 151 Zinc; catalytic (Probable).
 MOD_RES 2 2 N-acetylalanine (By similarity).  

Keyword

 Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Metal-binding; Metalloprotease; Nucleus; Protease; Reference proteome; Signalosome; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 334 AA 

Protein Sequence

Gene Ontology

 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0008180; C:signalosome; IDA:MGI.
 GO:0005667; C:transcription factor complex; TAS:MGI.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0008237; F:metallopeptidase activity; IEA:UniProtKB-KW.
 GO:0003713; F:transcription coactivator activity; TAS:MGI.
 GO:0010388; P:cullin deneddylation; IEA:Compara.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
 GO:0051726; P:regulation of cell cycle; IMP:MGI.
 GO:0046328; P:regulation of JNK cascade; IEA:Compara.
 GO:0006355; P:regulation of transcription, DNA-dependent; TAS:MGI. 

Interpro

 IPR000555; JAB1_Mov34_MPN_PAD1. 

Pfam

 PF01398; JAB 

SMART

 SM00232; JAB_MPN 

PROSITE

  

PRINTS