UniProt Accession

 RAC2_HUMAN; P15153; Q9UDJ4 

Genbank Protein ID

 M29871; AF498965; CR456555; BT006919; Z82188; BC001485; M64595 

Genbank Nucleotide ID

 AAA36538.1; AAM21112.1; CAG30441.1; AAP35565.1; CAB45265.1; AAH01485.1; AAA35941.1 

Protein Name

 Ras-related C3 botulinum toxin substrate 2 

Protein Synonyms/Alias

 GX; Small G protein; p21-Rac2 

Gene Name

 RAC2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
5	***MQAIKCVVVGDG	ubiquitination	[1]
96	SYENVRAKWFPEVRH	ubiquitination	[1, 2]
116	PIILVGTKLDLRDDK	ubiquitination	[1]
123	KLDLRDDKDTIEKLK	ubiquitination	[1, 3]
133	IEKLKEKKLAPITYP	ubiquitination	[1, 2]
147	PQGLALAKEIDSVKY	acetylation	[4]
147	PQGLALAKEIDSVKY	ubiquitination	[1, 2]
153	AKEIDSVKYLECSAL	ubiquitination	[1]
166	ALTQRGLKTVFDEAI	ubiquitination	[1, 3]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 

Functional Description

 Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Augments the production of reactive oxygen species (ROS) by NADPH oxidase. 

Sequence Annotation

 NP_BIND 10 17 GTP (By similarity).
 NP_BIND 57 61 GTP (By similarity).
 NP_BIND 115 118 GTP (By similarity).
 MOTIF 32 40 Effector region (Potential).
 MOD_RES 39 39 ADP-ribosylasparagine; by botulinum toxin
 MOD_RES 147 147 N6-acetyllysine.
 MOD_RES 189 189 Cysteine methyl ester (Probable).
 LIPID 189 189 S-geranylgeranyl cysteine.  

Keyword

 3D-structure; Acetylation; ADP-ribosylation; Complete proteome; Cytoplasm; Direct protein sequencing; Disease mutation; GTP-binding; Lipoprotein; Methylation; Nucleotide-binding; Polymorphism; Prenylation; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 192 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005635; C:nuclear envelope; IEA:Compara.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; TAS:ProtInc.
 GO:0030036; P:actin cytoskeleton organization; IEA:Compara.
 GO:0007411; P:axon guidance; TAS:Reactome.
 GO:0045453; P:bone resorption; IEA:Compara.
 GO:0030031; P:cell projection assembly; IEA:Compara.
 GO:0030168; P:platelet activation; TAS:Reactome.
 GO:0008284; P:positive regulation of cell proliferation; IEA:Compara.
 GO:0010310; P:regulation of hydrogen peroxide metabolic process; TAS:BHF-UCL.
 GO:0060263; P:regulation of respiratory burst; IDA:BHF-UCL.
 GO:0051056; P:regulation of small GTPase mediated signal transduction; TAS:Reactome.
 GO:0007264; P:small GTPase mediated signal transduction; TAS:Reactome. 

Interpro

 IPR027417; P-loop_NTPase.
 IPR005225; Small_GTP-bd_dom.
 IPR001806; Small_GTPase.
 IPR003578; Small_GTPase_Rho. 

Pfam

 PF00071; Ras 

SMART

 SM00174; RHO 

PROSITE

 PS51420; RHO 

PRINTS

 PR00449; RASTRNSFRMNG.