Tag | Content |
---|
CPLM ID | CPLM-012027 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Serine/threonine-protein kinase 3 |
Protein Synonyms/Alias | Mammalian STE20-like protein kinase 2; MST-2; STE20-like kinase MST2; Serine/threonine-protein kinase Krs-1; Serine/threonine-protein kinase 3 36kDa subunit; MST2/N; Serine/threonine-protein kinase 3 20kDa subunit; MST2/C |
Gene Name | STK3 |
Gene Synonyms/Alias | KRS1; MST2 |
Created Date | July 27, 2013 |
Organism | Homo sapiens (Human) |
NCBI Taxa ID | 9606 |
Lysine Modification | Position | Peptide | Type | References |
---|
21 | LSEDSLTKQPEEVFD | ubiquitination | [1] | 56 | SGQVVAIKQVPVESD | ubiquitination | [1] | 177 | QLTDTMAKRNTVIGT | ubiquitination | [1] | 243 | NPPPTFRKPELWSDD | ubiquitination | [2] | 256 | DDFTDFVKKCLVKNP | ubiquitination | [1] | 298 | ITEAMEIKAKRHEEQ | ubiquitination | [1] |
|
Reference | [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization. Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW. Nature. 2013 Apr 18;496(7445):372-6. [ PMID: 23503661] [2] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin. Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI. J Proteome Res. 2012 Feb 3;11(2):796-807. [ PMID: 22053931] |
Functional Description | Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1 (By similarity). Phosphorylates NEK2 and plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosome, and its ability to phosphorylate CROCC and CEP250. In conjunction with SAV1, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation. Positively regulates RAF1 activation via suppression of the inhibitory phosphorylation of RAF1 on 'Ser-259'. Phosphorylates MOBKL1A and RASSF2. Phosphorylates MOBKL1B on 'Thr-74'. Acts cooperatively with MOBKL1B to activate STK38. |
Sequence Annotation | DOMAIN 27 278 Protein kinase. DOMAIN 437 484 SARAH. NP_BIND 33 41 ATP (By similarity). ACT_SITE 146 146 Proton acceptor (By similarity). BINDING 56 56 ATP. MOD_RES 1 1 N-acetylmethionine. MOD_RES 15 15 Phosphoserine. MOD_RES 117 117 Phosphothreonine; by PKB/AKT1. MOD_RES 180 180 Phosphothreonine; by autocatalysis MOD_RES 316 316 Phosphoserine. MOD_RES 336 336 Phosphothreonine. MOD_RES 384 384 Phosphothreonine; by PKB/AKT1. MOD_RES 444 444 Phosphoserine. |
Keyword | Acetylation; Apoptosis; ATP-binding; Coiled coil; Complete proteome; Cytoplasm; Direct protein sequencing; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 491 AA |
Protein Sequence | MEQPPAPKSK LKKLSEDSLT KQPEEVFDVL EKLGEGSYGS VFKAIHKESG QVVAIKQVPV 60 ESDLQEIIKE ISIMQQCDSP YVVKYYGSYF KNTDLWIVME YCGAGSVSDI IRLRNKTLIE 120 DEIATILKST LKGLEYLHFM RKIHRDIKAG NILLNTEGHA KLADFGVAGQ LTDTMAKRNT 180 VIGTPFWMAP EVIQEIGYNC VADIWSLGIT SIEMAEGKPP YADIHPMRAI FMIPTNPPPT 240 FRKPELWSDD FTDFVKKCLV KNPEQRATAT QLLQHPFIKN AKPVSILRDL ITEAMEIKAK 300 RHEEQQRELE EEEENSDEDE LDSHTMVKTS VESVGTMRAT STMSEGAQTM IEHNSTMLES 360 DLGTMVINSE DEEEEDGTMK RNATSPQVQR PSFMDYFDKQ DFKNKSHENC NQNMHEPFPM 420 SKNVFPDNWK VPQDGDFDFL KNLSLEELQM RLKALDPMME REIEELRQRY TAKRQPILDA 480 MDAKKRRQQN F 491 |
Gene Ontology | GO:0005829; C:cytosol; TAS:Reactome. GO:0005634; C:nucleus; IEA:UniProtKB-SubCell. GO:0005524; F:ATP binding; IDA:UniProtKB. GO:0000287; F:magnesium ion binding; IDA:UniProtKB. GO:0046983; F:protein dimerization activity; ISS:UniProtKB. GO:0043539; F:protein serine/threonine kinase activator activity; IDA:BHF-UCL. GO:0004674; F:protein serine/threonine kinase activity; IDA:UniProtKB. GO:0006915; P:apoptotic process; TAS:UniProtKB. GO:0035329; P:hippo signaling cascade; TAS:UniProtKB. GO:0007243; P:intracellular protein kinase cascade; IDA:UniProtKB. GO:0090090; P:negative regulation of canonical Wnt receptor signaling pathway; IMP:BHF-UCL. GO:0043065; P:positive regulation of apoptotic process; ISS:UniProtKB. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |