CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004429
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cyclic AMP-responsive element-binding protein 1 
Protein Synonyms/Alias
 CREB-1; cAMP-responsive element-binding protein 1 
Gene Name
 CREB1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
91QTVQSSCKDLKRLFSacetylation[1]
94QSSCKDLKRLFSGTQacetylation[1]
136SRRPSYRKILNDLSSacetylation[1]
136SRRPSYRKILNDLSSubiquitination[2, 3]
285PAEEAARKREVRLMKsumoylation[4]
304ARECRRKKKEYVKCLsumoylation[4]
330KTLIEELKALKDLYCubiquitination[2, 3, 5]
333IEELKALKDLYCHKSubiquitination[5]
339LKDLYCHKSD*****ubiquitination[5]
Reference
 [1] Acetylation of cAMP-responsive element-binding protein (CREB) by CREB-binding protein enhances CREB-dependent transcription.
 Lu Q, Hutchins AE, Doyle CM, Lundblad JR, Kwok RP.
 J Biol Chem. 2003 May 2;278(18):15727-34. [PMID: 12595525]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Small ubiquitin-related modifier-1 modification mediates resolution of CREB-dependent responses to hypoxia.
 Comerford KM, Leonard MO, Karhausen J, Carey R, Colgan SP, Taylor CT.
 Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):986-91. [PMID: 12552083]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells. 
Sequence Annotation
 DOMAIN 101 160 KID.
 DOMAIN 283 341 bZIP.
 REGION 284 309 Basic motif (By similarity).
 REGION 311 332 Leucine-zipper (By similarity).
 MOD_RES 133 133 Phosphoserine; by CaMK1, CaMK2, CaMK4,
 MOD_RES 142 142 Phosphoserine; by CaMK2 (By similarity).
 MOD_RES 271 271 Phosphoserine; by HIPK2.
 CROSSLNK 285 285 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 304 304 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 3D-structure; Activator; Alternative splicing; Chromosomal rearrangement; Complete proteome; Differentiation; DNA-binding; Host-virus interaction; Isopeptide bond; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transcription; Transcription regulation; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 341 AA 
Protein Sequence
MTMESGAENQ QSGDAAVTEA ENQQMTVQAQ PQIATLAQVS MPAAHATSSA PTVTLVQLPN 60
GQTVQVHGVI QAAQPSVIQS PQVQTVQSSC KDLKRLFSGT QISTIAESED SQESVDSVTD 120
SQKRREILSR RPSYRKILND LSSDAPGVPR IEEEKSEEET SAPAITTVTV PTPIYQTSSG 180
QYIAITQGGA IQLANNGTDG VQGLQTLTMT NAAATQPGTT ILQYAQTTDG QQILVPSNQV 240
VVQAASGDVQ TYQIRTAPTS TIAPGVVMAS SPALPTQPAE EAARKREVRL MKNREAAREC 300
RRKKKEYVKC LENRVAVLEN QNKTLIEELK ALKDLYCHKS D 341 
Gene Ontology
 GO:0005719; C:nuclear euchromatin; IDA:BHF-UCL.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0005667; C:transcription factor complex; IEA:Compara.
 GO:0035497; F:cAMP response element binding; IDA:BHF-UCL.
 GO:0000980; F:RNA polymerase II distal enhancer sequence-specific DNA binding; IDA:BHF-UCL.
 GO:0003705; F:RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity; IDA:BHF-UCL.
 GO:0001190; F:RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription; IDA:BHF-UCL.
 GO:0003712; F:transcription cofactor activity; TAS:ProtInc.
 GO:0007202; P:activation of phospholipase C activity; TAS:Reactome.
 GO:0007411; P:axon guidance; TAS:Reactome.
 GO:0007173; P:epidermal growth factor receptor signaling pathway; TAS:Reactome.
 GO:0008543; P:fibroblast growth factor receptor signaling pathway; TAS:Reactome.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0007595; P:lactation; IEA:Compara.
 GO:0002755; P:MyD88-dependent toll-like receptor signaling pathway; TAS:Reactome.
 GO:0010944; P:negative regulation of transcription by competitive promoter binding; IDA:BHF-UCL.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0048015; P:phosphatidylinositol-mediated signaling; TAS:Reactome.
 GO:0021983; P:pituitary gland development; IEA:Compara.
 GO:0045600; P:positive regulation of fat cell differentiation; ISS:UniProtKB.
 GO:0046887; P:positive regulation of hormone secretion; IEA:Compara.
 GO:0046889; P:positive regulation of lipid biosynthetic process; ISS:UniProtKB.
 GO:0040018; P:positive regulation of multicellular organism growth; IEA:Compara.
 GO:0006468; P:protein phosphorylation; IDA:MGI.
 GO:0050821; P:protein stabilization; ISS:UniProtKB.
 GO:0008361; P:regulation of cell size; IEA:Compara.
 GO:0042493; P:response to drug; IEA:Compara.
 GO:0051403; P:stress-activated MAPK cascade; TAS:Reactome.
 GO:0007268; P:synaptic transmission; TAS:Reactome.
 GO:0034166; P:toll-like receptor 10 signaling pathway; TAS:Reactome.
 GO:0034134; P:toll-like receptor 2 signaling pathway; TAS:Reactome.
 GO:0034138; P:toll-like receptor 3 signaling pathway; TAS:Reactome.
 GO:0034142; P:toll-like receptor 4 signaling pathway; TAS:Reactome.
 GO:0034146; P:toll-like receptor 5 signaling pathway; TAS:Reactome.
 GO:0034162; P:toll-like receptor 9 signaling pathway; TAS:Reactome.
 GO:0038123; P:toll-like receptor TLR1:TLR2 signaling pathway; TAS:Reactome.
 GO:0038124; P:toll-like receptor TLR6:TLR2 signaling pathway; TAS:Reactome.
 GO:0035666; P:TRIF-dependent toll-like receptor signaling pathway; TAS:Reactome.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 
Interpro
 IPR004827; bZIP.
 IPR003102; Coactivator_CBP_pKID.
 IPR001630; Leuzip_CREB. 
Pfam
 PF00170; bZIP_1
 PF02173; pKID 
SMART
 SM00338; BRLZ 
PROSITE
 PS50217; BZIP
 PS00036; BZIP_BASIC
 PS50953; KID 
PRINTS
 PR00041; LEUZIPPRCREB.