| Tag | Content |
|---|
| CPLM ID | CPLM-000493 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Cytochrome P450 98A3 |
Protein Synonyms/Alias | Protein REDUCED EPIDERMAL FLUORESCENCE 8; p-coumaroylshikimate/quinate 3'-hydrolxylase; C3'H |
Gene Name | CYP98A3 |
Gene Synonyms/Alias | C3'H; REF8; At2g40890; T20B5.9 |
Created Date | July 27, 2013 |
Organism | Arabidopsis thaliana (Mouse-ear cress) |
NCBI Taxa ID | 3702 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 172 | AKGLQLRKYLGAVAF | ubiquitination | [1] | | 189 | ITRLAFGKRFMNAEG | ubiquitination | [1] |
|
Reference | [1] Multidimensional protein identification technology (MudPIT) analysis of ubiquitinated proteins in plants. Maor R, Jones A, Nühse TS, Studholme DJ, Peck SC, Shirasu K. Mol Cell Proteomics. 2007 Apr;6(4):601-10. [ PMID: 17272265] |
Functional Description | Cytochrome P450 which catalyzes 3'-hydroxylation of p- coumaric esters of shikimic/quinic acids to form lignin monomers. Can use p-coumarate, p-coumaraldehyde, p-coumaroyl methyl ester, 5-O-(4-coumaroyl) D-quinate and 5-O-(4-coumaroyl) shikimate as substrates, but not p-coumaryl alcohol, p-coumaroyl CoA, 1-O-p- coumaroyl-beta-D-glucose, p-hydroxy-cinnamyl alcohol, cinnamate, caffeate or ferulate. Has a weak activity on tri(p- coumaroyl)spermidine, but none on triferuloylspermidine. Hydroxylates preferentially the 5-O-isomer, but can also convert the 4-O- and 3-O-isomers with a lower efficiency. Involved in the biosynthesis of the coumarins scopoletin and scopolin. Essential for the biosynthesis of lignin. |
Sequence Annotation | METAL 438 438 Iron (heme axial ligand) (By similarity).
CROSSLNK 172 172 Glycyl lysine isopeptide (Lys-Gly)
CROSSLNK 189 189 Glycyl lysine isopeptide (Lys-Gly) |
Keyword | Complete proteome; Heme; Iron; Isopeptide bond; Membrane; Metal-binding; Monooxygenase; Oxidoreductase; Reference proteome; Transmembrane; Transmembrane helix; Ubl conjugation. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 508 AA |
Protein Sequence | MSWFLIAVAT IAAVVSYKLI QRLRYKFPPG PSPKPIVGNL YDIKPVRFRC YYEWAQSYGP 60
IISVWIGSIL NVVVSSAELA KEVLKEHDQK LADRHRNRST EAFSRNGQDL IWADYGPHYV 120
KVRKVCTLEL FTPKRLESLR PIREDEVTAM VESVFRDCNL PENRAKGLQL RKYLGAVAFN 180
NITRLAFGKR FMNAEGVVDE QGLEFKAIVS NGLKLGASLS IAEHIPWLRW MFPADEKAFA 240
EHGARRDRLT RAIMEEHTLA RQKSSGAKQH FVDALLTLKD QYDLSEDTII GLLWDMITAG 300
MDTTAITAEW AMAEMIKNPR VQQKVQEEFD RVVGLDRILT EADFSRLPYL QCVVKESFRL 360
HPPTPLMLPH RSNADVKIGG YDIPKGSNVH VNVWAVARDP AVWKNPFEFR PERFLEEDVD 420
MKGHDFRLLP FGAGRRVCPG AQLGINLVTS MMSHLLHHFV WTPPQGTKPE EIDMSENPGL 480
VTYMRTPVQA VATPRLPSDL YKRVPYDM 508 |
Gene Ontology | GO:0005783; C:endoplasmic reticulum; IDA:TAIR. GO:0016021; C:integral to membrane; IEA:UniProtKB-KW. GO:0005739; C:mitochondrion; IDA:TAIR. GO:0005886; C:plasma membrane; IDA:TAIR. GO:0009055; F:electron carrier activity; IEA:InterPro. GO:0020037; F:heme binding; IEA:InterPro. GO:0005506; F:iron ion binding; IEA:InterPro. GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro. GO:0046409; F:p-coumarate 3-hydroxylase activity; IDA:TAIR. GO:0009805; P:coumarin biosynthetic process; IMP:TAIR. GO:0009813; P:flavonoid biosynthetic process; IMP:TAIR. GO:0009809; P:lignin biosynthetic process; IMP:TAIR. |
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Pfam | |
SMART | |
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PRINTS | |