UniProt Accession

 CDC53_YEAST; Q12018; D6VRL7 

Genbank Protein ID

 U43564; X96876; Z74180; BK006938 

Genbank Nucleotide ID

 AAB38821.1; CAA65628.1; CAA98702.1; DAA11727.1 

Protein Name

 Cell division control protein 53 

Protein Synonyms/Alias

 Cullin-A; E3 ubiquitin ligase complex SCF subunit CDC53 

Gene Name

 CDC53 

Gene Synonyms/Alias

 YDL132W; D2190 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
276	IYWDDHTKKPLSMAL	ubiquitination	[1]
277	YWDDHTKKPLSMALN	ubiquitination	[1]

Reference

 [1] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269] 

Functional Description

 Core component of multiple cullin-RING-based SCF (SKP1- CUL1-F-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The SCF complex associates with CDC34 as the E2 ubiquitin- conjugating enzyme. The functional specificity of the SCF complex depends on the type of F-box protein. SCF(CDC4) controls the G1- to-S phase transition; it directs ubiquitination of the phosphorylated CDK inhibitor SIC1 and of CDC6. SCF(CDC4) directs ubiquitination of GCN4. SCF(GRR1) directs ubiquitination of phosphorylated CLN1, CLN2 and GIC2. SCF(MET30) directs ubiquitination of MET4. SCF(DIA2) is specifically involved in the pheromone induced degradation of phosphorylated TEC1. SCF(MDM30) seems to direct ubiquitination of FZ01. Involved in the regulation of methionine biosynthesis genes. 

Sequence Annotation

 REGION 9 280 Required for interaction with SKP1/CBF3D
 REGION 448 748 Required for interaction with CDC34/UBC3.
 CROSSLNK 760 760 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 3D-structure; Cell cycle; Cell division; Complete proteome; Cytoplasm; Isopeptide bond; Nucleus; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 815 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0019005; C:SCF ubiquitin ligase complex; IDA:SGD.
 GO:0003688; F:DNA replication origin binding; IPI:SGD.
 GO:0030674; F:protein binding, bridging; IMP:SGD.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0000082; P:G1/S transition of mitotic cell cycle; IMP:SGD.
 GO:0000086; P:G2/M transition of mitotic cell cycle; IGI:SGD.
 GO:0042787; P:protein ubiquitination involved in ubiquitin-dependent protein catabolic process; IDA:SGD.
 GO:0031146; P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; IDA:SGD. 

Interpro

 IPR016157; Cullin_CS.
 IPR016158; Cullin_homology.
 IPR001373; Cullin_N.
 IPR019559; Cullin_neddylation_domain.
 IPR016159; Cullin_repeat-like_dom.
 IPR011991; WHTH_DNA-bd_dom. 

Pfam

 PF00888; Cullin
 PF10557; Cullin_Nedd8 

SMART

 SM00182; CULLIN
 SM00884; Cullin_Nedd8 

PROSITE

 PS01256; CULLIN_1
 PS50069; CULLIN_2 

PRINTS