CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024354
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 
Protein Synonyms/Alias
 Phosphoinositide phospholipase C-eta-2; Phosphoinositide phospholipase C-like 4; PLC-L4; Phospholipase C-like protein 4; Phospholipase C-eta-2; PLC-eta2 
Gene Name
 Plch2 
Gene Synonyms/Alias
 Plcl4 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
479DVIETINKYAFIKNEubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023
Functional Description
 The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. This phospholipase activity is very sensitive to calcium. May be important for formation and maintenance of the neuronal network in the postnatal brain. 
Sequence Annotation
 DOMAIN 121 229 PH.
 DOMAIN 243 278 EF-hand 1.
 DOMAIN 279 315 EF-hand 2.
 DOMAIN 400 545 PI-PLC X-box.
 DOMAIN 707 821 PI-PLC Y-box.
 DOMAIN 826 933 C2.
 REGION 75 229 Necessary for plasma membrane
 ACT_SITE 415 415 By similarity.
 ACT_SITE 459 459 By similarity.
 METAL 416 416 Calcium 1; catalytic (By similarity).
 METAL 445 445 Calcium 1; catalytic (By similarity).
 METAL 447 447 Calcium 1; catalytic (By similarity).
 METAL 494 494 Calcium 1; catalytic (By similarity).
 METAL 865 865 Calcium 2; via carbonyl oxygen (By
 METAL 867 867 Calcium 2 (By similarity).
 METAL 891 891 Calcium 2 (By similarity).
 METAL 920 920 Calcium 3 (By similarity).
 METAL 921 921 Calcium 3; via carbonyl oxygen (By
 METAL 922 922 Calcium 3 (By similarity).
 BINDING 543 543 Substrate (By similarity).
 BINDING 545 545 Substrate (By similarity).
 BINDING 734 734 Substrate (By similarity).
 BINDING 761 761 Substrate (By similarity).
 MOD_RES 676 676 Phosphoserine.
 MOD_RES 686 686 Phosphoserine.  
Keyword
 Alternative splicing; Calcium; Cell membrane; Complete proteome; Cytoplasm; Hydrolase; Lipid degradation; Lipid metabolism; Membrane; Metal-binding; Phosphoprotein; Reference proteome; Repeat; Transducer. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1501 AA 
Protein Sequence
MGGLAWGPSR AAGSSWVNAS GTWEQPLRGF SGLQGGRRRG RGEKGIPEEP LCQLTPQLGL 60
SLRVPFGLGD YGLDMPGPQP SAASQTTGAV ACLAEVLLWV GGSVVVSPRW QLSLVVERCM 120
SAMQEGTQMV KLRGSSKGLV RFYYLDEHRS CLRWRPSRKN EKAKISIDSI QEVSEGRQSE 180
IFQRYPDSSF DPNCCFSIYH GSHRESLDLV SPSSEEARTW VTGLRYLMAG ISDEDSLARR 240
QRTRDQWLKQ TFDEADKNGD GSLSISEVLQ LLHKLNVNLP RQRVKQMFRE ADTDDHQGTL 300
GFEEFCAFYK MMSTRRDLYL LMLTYSNHKD HLDASDLQRF LEVEQKMNGV TLESCQNIIE 360
QFEPCLENKS KGMLGIDGFT NYTRSPAGDI FNPEHNRVHQ DMTQPLSHYF ITSSHNTYLV 420
GDQLMSQSRV DMYAWVLQAG CRCVEVDCWD GPDGEPIVHH GYTLTSKILF KDVIETINKY 480
AFIKNEYPVI LSIENHCSVV QQKKMAQYLT DILGDKLDLS SVSSEDATML PSPQMLKGKI 540
LVKGKKLPAN ISEDAEEGEV SDEDSADEME DDCKLLNGDA STNRKRVENI AKKKLDSLIK 600
ESKIRDCEDP NDFSVSTLSP SGKLGRKAEA KKGQSKVEED VEAGEDSGVS RQNSRLFMSS 660
FSKRKKKGSK IKKVASVEEG DETLDSPGSQ SRGTARQKKT MKLSRALSDL VKYTKSVGTH 720
DVEIEVVSSW QVSSFSETKA HQILQQKPTQ YLRFNQHQLS RIYPSSYRVD SSNYNPQPFW 780
NAGCQMVALN YQSEGRMLQL NRAKFSANGD CGYVLKPQCM CQGVFNPNSE DPLPGQLKKQ 840
LALRIISGQQ LPKPRDSVLG DRGEIIDPFV EVEVIGLPVD CSKEQTRVVD DNGFNPMWEE 900
TLVFTVHMPE IALVRFLVWD HDPIGRDFIG QRTLAFSSIM PGYRHVYLEG MEEASIFVHV 960
AVSDISGKVK QTLGLKGLFL RGTKPGSLDS HAAGQPLPRP SVSQRLLRRT ASAPTKSQKP 1020
SRKGFPELAL GTQDAGSEGA ADDVAPSSPN PALEAPTQER SGSSSPRGKA PGGEATEERT 1080
LAQVRSPNAP EGPGPAGMAA TCMKCVVGSC AGMDVEGLRR EQQPSPGPAG SHMAISHQPR 1140
ARVDSLGGPC CSPSPRATPG RSKEAPKGPR ARRQGPGGGS VSSDSSSPDS PGSPKVAPCQ 1200
PEGAHRQQGA LQGEMNALFV QKLEEIRSHS PMFSTVRD 1238 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IDA:MGI.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0004435; F:phosphatidylinositol phospholipase C activity; IEA:EC.
 GO:0004629; F:phospholipase C activity; IDA:MGI.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0004871; F:signal transducer activity; IEA:UniProtKB-KW.
 GO:0035556; P:intracellular signal transduction; IEA:InterPro.
 GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
 GO:0046488; P:phosphatidylinositol metabolic process; IDA:MGI. 
Interpro
 IPR000008; C2_Ca-dep.
 IPR008973; C2_Ca/lipid-bd_dom_CaLB.
 IPR018029; C2_membr_targeting.
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR011993; PH_like_dom.
 IPR001192; Pinositol_PLipase_C.
 IPR017946; PLC-like_Pdiesterase_TIM-brl.
 IPR001849; Pleckstrin_homology.
 IPR015359; PLipase_C_EF-hand-like.
 IPR000909; PLipase_C_PInositol-sp_X_dom.
 IPR001711; PLipase_C_Pinositol-sp_Y. 
Pfam
 PF00168; C2
 PF13499; EF_hand_5
 PF09279; efhand_like
 PF00388; PI-PLC-X
 PF00387; PI-PLC-Y 
SMART
 SM00239; C2
 SM00054; EFh
 SM00233; PH
 SM00148; PLCXc
 SM00149; PLCYc 
PROSITE
 PS50004; C2
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS50003; PH_DOMAIN
 PS50007; PIPLC_X_DOMAIN
 PS50008; PIPLC_Y_DOMAIN 
PRINTS
 PR00390; PHPHLIPASEC.