CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022345
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ganglioside-induced differentiation-associated protein 2 
Protein Synonyms/Alias
  
Gene Name
 GDAP2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
50RSPFLYNKDVNGKVVubiquitination[1, 2]
217LEEGTYQKLLPLYFPubiquitination[3]
296RMEGDIDKQRKLILQubiquitination[1]
299GDIDKQRKLILQGQLubiquitination[4, 5]
313LSEAALQKQHQRNYNubiquitination[1, 2, 4, 5, 6, 7]
338LSDIASLKALYQTGVubiquitination[1, 2, 3, 7, 8, 9, 10]
408LDSDFLKKLYDVVDVubiquitination[7]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [7] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [8] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [9] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [10] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
 DOMAIN 43 223 Macro.
 DOMAIN 333 481 CRAL-TRIO.  
Keyword
 Alternative splicing; Complete proteome; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 497 AA 
Protein Sequence
MDPLGAPSQF VDVDTLPSWG DSCQDELNSS DTTAEIFQED TVRSPFLYNK DVNGKVVLWK 60
GDVALLNCTA IVNTSNESLT DKNPVSESIF MLAGPDLKED LQKLKGCRTG EAKLTKGFNL 120
AARFIIHTVG PKYKSRYRTA AESSLYSCYR NVLQLAKEQS MSSVGFCVIN SAKRGYPLED 180
ATHIALRTVR RFLEIHGETI EKVVFAVSDL EEGTYQKLLP LYFPRSLKEE NRSLPYLPAD 240
IGNAEGEPVV PERQIRISEK PGAPEDNQEE EDEGLGVDLS FIGSHAFARM EGDIDKQRKL 300
ILQGQLSEAA LQKQHQRNYN RWLCQARSED LSDIASLKAL YQTGVDNCGR TVMVVVGRNI 360
PVTLIDMDKA LLYFIHVMDH IAVKEYVLVY FHTLTSEYNH LDSDFLKKLY DVVDVKYKRN 420
LKAVYFVHPT FRSKVSTWFF TTFSVSGLKD KIHHVDSLHQ LFSAISPEQI DFPPFVLEYD 480
ARENGPYYTS YPPSPDL 497 
Gene Ontology
 GO:0032526; P:response to retinoic acid; IEA:Compara. 
Interpro
 IPR002589; A1pp.
 IPR001251; CRAL-TRIO_dom. 
Pfam
 PF01661; Macro 
SMART
 SM00516; SEC14 
PROSITE
 PS50191; CRAL_TRIO
 PS51154; MACRO 
PRINTS