UniProt Accession

 NUOB_ECOLI; P0AFC7; P33598; P78090; P78186; P78187 

Genbank Protein ID

 X68301; U00096; AP009048 

Genbank Nucleotide ID

 CAA48361.1; AAC75347.1; BAA16121.2 

Protein Name

 NADH-quinone oxidoreductase subunit B 

Protein Synonyms/Alias

 NADH dehydrogenase I subunit B; NDH-1 subunit B; NUO2 

Gene Name

 nuoB 

Gene Synonyms/Alias

 b2287; JW5875 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
21	NDRYPLQKQEIVTDP	acetylation	[1]
35	PLEQEVNKNVFMGKL	acetylation	[1]
41	NKNVFMGKLNDMVNW	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. 

Sequence Annotation

 METAL 63 63 Iron-sulfur (4Fe-4S) (Potential).
 METAL 64 64 Iron-sulfur (4Fe-4S) (Potential).
 METAL 129 129 Iron-sulfur (4Fe-4S) (Potential).
 METAL 158 158 Iron-sulfur (4Fe-4S) (Potential).  

Keyword

 4Fe-4S; Cell inner membrane; Cell membrane; Complete proteome; Direct protein sequencing; Iron; Iron-sulfur; Membrane; Metal-binding; NAD; Oxidoreductase; Quinone; Reference proteome; Transport; Ubiquinone. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 220 AA 

Protein Sequence

Gene Ontology

 GO:0045272; C:plasma membrane respiratory chain complex I; IDA:EcoCyc.
 GO:0051539; F:4 iron, 4 sulfur cluster binding; IMP:EcoCyc.
 GO:0005506; F:iron ion binding; IEA:HAMAP.
 GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IMP:EcoCyc.
 GO:0048038; F:quinone binding; IEA:UniProtKB-KW.
 GO:0009060; P:aerobic respiration; IMP:EcoCyc.
 GO:0015990; P:electron transport coupled proton transport; IMP:EcoCyc.
 GO:0019684; P:photosynthesis, light reaction; IEA:HAMAP. 

Interpro

 IPR006137; NADH_UbQ_OxRdtase-like_20kDa.
 IPR006138; NADH_UQ_OxRdtase_20Kd_su.
 IPR014406; NiFe-hyd_3_ssu/Q_oxred_NuoB. 

Pfam

 PF01058; Oxidored_q6 

SMART

  

PROSITE

 PS01150; COMPLEX1_20K 

PRINTS