CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008991
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Puromycin-sensitive aminopeptidase 
Protein Synonyms/Alias
 PSA; Cytosol alanyl aminopeptidase; AAP-S 
Gene Name
 NPEPPS 
Gene Synonyms/Alias
 PSA 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
155FVGELNDKMKGFYRSubiquitination[1, 2, 3]
208DISLVVPKDRVALSNubiquitination[1, 3]
222NMNVIDRKPYPDDENubiquitination[2]
234DENLVEVKFARTPVMubiquitination[1, 2, 3]
274RVYTPVGKAEQGKFAubiquitination[2, 4]
279VGKAEQGKFALEVAAubiquitination[4]
287FALEVAAKTLPFYKDubiquitination[1, 2, 3]
293AKTLPFYKDYFNVPYubiquitination[1, 2, 3]
440FDAISYSKGASVIRMubiquitination[2]
455LHDYIGDKDFKKGMNubiquitination[1, 3]
467GMNMYLTKFQQKNAAubiquitination[1, 3]
558DPNQAKLKILMDKPEubiquitination[4]
736FKDHVEGKQILSADLubiquitination[4]
829HGRKAAWKFIKDNWEubiquitination[1, 3]
832KAAWKFIKDNWEELYubiquitination[4]
863VEGFAVDKMAGEVKAubiquitination[4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Aminopeptidase with broad substrate specificity for several peptides. Involved in proteolytic events essential for cell growth and viability. May act as regulator of neuropeptide activity. Plays a role in the antigen-processing pathway for MHC class I molecules. Involved in the N-terminal trimming of cytotoxic T-cell epitope precursors. Digests the poly-Q peptides found in many cellular proteins. Digests tau from normal brain more efficiently than tau from Alzheimer disease brain. 
Sequence Annotation
 REGION 316 320 Substrate binding (By similarity).
 MOTIF 726 730 Nuclear localization signal (Potential).
 ACT_SITE 353 353 Proton acceptor (By similarity).
 METAL 352 352 Zinc; catalytic (By similarity).
 METAL 356 356 Zinc; catalytic (By similarity).
 METAL 375 375 Zinc; catalytic (By similarity).
 BINDING 180 180 Substrate (By similarity).
 MOD_RES 464 464 Nitrated tyrosine (By similarity).  
Keyword
 Aminopeptidase; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Metal-binding; Metalloprotease; Nitration; Nucleus; Protease; Reference proteome; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 919 AA 
Protein Sequence
MWLAAAAPSL ARRLLFLGPP PPPLLLLVFS RSSRRRLHSL GLAAMPEKRP FERLPADVSP 60
INYSLCLKPD LLDFTFEGKL EAAAQVRQAT NQIVMNCADI DIITASYAPE GDEEIHATGF 120
NYQNEDEKVT LSFPSTLQTG TGTLKIDFVG ELNDKMKGFY RSKYTTPSGE VRYAAVTQFE 180
ATDARRAFPC WDEPAIKATF DISLVVPKDR VALSNMNVID RKPYPDDENL VEVKFARTPV 240
MSTYLVAFVV GEYDFVETRS KDGVCVRVYT PVGKAEQGKF ALEVAAKTLP FYKDYFNVPY 300
PLPKIDLIAI ADFAAGAMEN WGLVTYRETA LLIDPKNSCS SSRQWVALVV GHELAHQWFG 360
NLVTMEWWTH LWLNEGFASW IEYLCVDHCF PEYDIWTQFV SADYTRAQEL DALDNSHPIE 420
VSVGHPSEVD EIFDAISYSK GASVIRMLHD YIGDKDFKKG MNMYLTKFQQ KNAATEDLWE 480
SLENASGKPI AAVMNTWTKQ MGFPLIYVEA EQVEDDRLLR LSQKKFCAGG SYVGEDCPQW 540
MVPITISTSE DPNQAKLKIL MDKPEMNVVL KNVKPDQWVK LNLGTVGFYR TQYSSAMLES 600
LLPGIRDLSL PPVDRLGLQN DLFSLARAGI ISTVEVLKVM EAFVNEPNYT VWSDLSCNLG 660
ILSTLLSHTD FYEEIQEFVK DVFSPIGERL GWDPKPGEGH LDALLRGLVL GKLGKAGHKA 720
TLEEARRRFK DHVEGKQILS ADLRSPVYLT VLKHGDGTTL DIMLKLHKQA DMQEEKNRIE 780
RVLGATLLPD LIQKVLTFAL SEEVRPQDTV SVIGGVAGGS KHGRKAAWKF IKDNWEELYN 840
RYQGGFLISR LIKLSVEGFA VDKMAGEVKA FFESHPAPSA ERTIQQCCEN ILLNAAWLKR 900
DAESIHQYLL QRKASPPTV 919 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0004177; F:aminopeptidase activity; TAS:ProtInc.
 GO:0008237; F:metallopeptidase activity; IEA:UniProtKB-KW.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0002474; P:antigen processing and presentation of peptide antigen via MHC class I; TAS:Reactome.
 GO:0071456; P:cellular response to hypoxia; IDA:UniProtKB.
 GO:0000209; P:protein polyubiquitination; TAS:Reactome.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW. 
Interpro
 IPR024571; DUF3358.
 IPR001930; Peptidase_M1.
 IPR014782; Peptidase_M1_N.
 IPR015568; Peptidase_M1_puromycin-sens. 
Pfam
 PF11838; DUF3358
 PF01433; Peptidase_M1 
SMART
  
PROSITE
 PS00142; ZINC_PROTEASE 
PRINTS
 PR00756; ALADIPTASE.