UniProt Accession

 CSTF1_HUMAN; Q05048; Q5QPD8 

Genbank Protein ID

 L02547; BT007138; AK312774; AL121914; AL121914; CH471077; CH471077; BC001011; BC007425 

Genbank Nucleotide ID

 AAA35691.1; AAP35802.1; BAG35638.1; CAC12718.2; CAI19328.1; EAW75548.1; EAW75549.1; AAH01011.1; AAH07425.1 

Protein Name

 Cleavage stimulation factor subunit 1 

Protein Synonyms/Alias

 CF-1 50 kDa subunit; Cleavage stimulation factor 50 kDa subunit; CSTF 50 kDa subunit; CstF-50 

Gene Name

 CSTF1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
143	DTERMLAKSAMPIEV	ubiquitination	[1]
198	GSRDYTLKLFDYSKP	ubiquitination	[2]
204	LKLFDYSKPSAKRAF	ubiquitination	[1, 2, 3]
208	DYSKPSAKRAFKYIQ	ubiquitination	[2]
326	KYILSSGKDSVAKLW	ubiquitination	[2]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. May be responsible for the interaction of CSTF with other factors to form a stable complex on the pre-mRNA. 

Sequence Annotation

 REPEAT 106 145 WD 1.
 REPEAT 171 210 WD 2.
 REPEAT 215 254 WD 3.
 REPEAT 260 301 WD 4.
 REPEAT 303 343 WD 5.
 REPEAT 395 430 WD 6.
 REGION 14 35 Hydrophobic.  

Keyword

 Complete proteome; Direct protein sequencing; mRNA processing; Nucleus; Reference proteome; Repeat; WD repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 431 AA 

Protein Sequence

Gene Ontology

 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0003723; F:RNA binding; TAS:ProtInc.
 GO:0006379; P:mRNA cleavage; TAS:ProtInc.
 GO:0006378; P:mRNA polyadenylation; TAS:ProtInc.
 GO:0000398; P:mRNA splicing, via spliceosome; TAS:Reactome.
 GO:0006369; P:termination of RNA polymerase II transcription; TAS:Reactome. 

Interpro

 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 

Pfam

 PF00400; WD40 

SMART

 SM00320; WD40 

PROSITE

 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 

PRINTS