CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009123
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Dynamin-like 120 kDa protein, mitochondrial 
Protein Synonyms/Alias
 Large GTP-binding protein; LargeG; Optic atrophy protein 1 homolog; Dynamin-like 120 kDa protein, form S1 
Gene Name
 Opa1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
243STSYAQQKRKVSDKEubiquitination[1]
265ELLHTQLKYQRILERacetylation[2]
633DRNELFEKAKNEILDacetylation[2]
871EVDPSLIKDTWHQVYacetylation[2]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123] 
Functional Description
 Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space. 
Sequence Annotation
 NP_BIND 295 302 GTP (Potential).
 NP_BIND 398 402 GTP (Potential).
 NP_BIND 467 470 GTP (Potential).
 MOD_RES 228 228 N6-acetyllysine (By similarity).
 MOD_RES 652 652 Phosphothreonine.
 MOD_RES 929 929 Phosphothreonine (By similarity).  
Keyword
 Acetylation; Alternative splicing; Apoptosis; Coiled coil; Complete proteome; GTP-binding; Hydrolase; Membrane; Mitochondrion; Mitochondrion inner membrane; Nucleotide-binding; Phosphoprotein; Reference proteome; Sensory transduction; Transit peptide; Transmembrane; Transmembrane helix; Vision. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 960 AA 
Protein Sequence
MWRAGRAAVA CEVCQSLVKH SSGIQRNVPL QKLHLVSRSI YRSHHPALKL QRPQLRTPFQ 60
QFSSLTHLSL HKLKLSPIKY GYQPRRNFWP ARLAARLLKL RYIILGSAVG GGYTAKKTFD 120
EWKDMIPDLS DYKWIVPDFI WEIDEYIDLE KIRKALPSSE DLASLAPDLD KITESLSLLK 180
DFFTAGSPGE TAFRATDHGS ESDKHYRKGL LGELILLQQQ IQEHEEEARR AAGQYSTSYA 240
QQKRKVSDKE KIDQLQEELL HTQLKYQRIL ERLEKENKEL RKLVLQKDDK GIHHRKLKKS 300
LIDMYSEVLD VLSDYDASYN TQDHLPRVVV VGDQSAGKTS VLEMIAQARI FPRGSGEMMT 360
RSPVKVTLSE GPHHVALFKD SSREFDLTKE EDLAALRHEI ELRMRKNVKE GCTVSPETIS 420
LNVKGPGLQR MVLVDLPGVI NTVTSGMAPD TKETIFSISK AYMQNPNAII LCIQDGSVDA 480
ERSIVTDLVS QMDPHGRRTI FVLTKVDLAE KNVASPSRIQ QIIEGKLFPM KALGYFAVVT 540
GKGNSSESIE AIREYEEEFF QNSKLLKTSM LKAHQVTTRN LSLAVSDCFW KMVRESVEQQ 600
ADSFKATRFN LETEWKNNYP RLRELDRNEL FEKAKNEILD EVISLSQVTP KHWEEILQQS 660
LWERVSTHVI ENIYLPAAQT MNSGTFNTTV DIKLKQWTDK QLPNKAVEVA WETLQEEFSR 720
FMTEPKGKEH DDIFDKLKEA VKEESIKRHK WNDFAEDSLR VIQHNALEDR SISDKQQWDA 780
AIYFMEEALQ GRLKDTENAI ENMIGPDWKK RWMYWKNRTQ EQCVHNETKN ELEKMLKVND 840
EHPAYLASDE ITTVRKNLES RGVEVDPSLI KDTWHQVYRR HFLKTALNHC NLCRRGFYYY 900
QRHFIDSELE CNDVVLFWRI QRMLAITANT LRQQLTNTEV RRLEKNVKEV LEDFAEDGEK 960
KVKLLTGKRV QLAEDLKKVR EIQEKLDAFI EALHQEK 997 
Gene Ontology
 GO:0030425; C:dendrite; IDA:UniProtKB.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0030061; C:mitochondrial crista; ISS:UniProtKB.
 GO:0005758; C:mitochondrial intermembrane space; IDA:UniProtKB.
 GO:0005741; C:mitochondrial outer membrane; ISS:UniProtKB.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; TAS:UniProtKB.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0007007; P:inner mitochondrial membrane organization; ISS:UniProtKB.
 GO:0008053; P:mitochondrial fusion; IDA:MGI.
 GO:2001243; P:negative regulation of intrinsic apoptotic signaling pathway; IMP:UniProtKB.
 GO:0090201; P:negative regulation of release of cytochrome c from mitochondria; IMP:UniProtKB.
 GO:0001843; P:neural tube closure; IMP:MGI.
 GO:0050896; P:response to stimulus; IEA:UniProtKB-KW.
 GO:0007601; P:visual perception; ISS:UniProtKB. 
Interpro
 IPR022812; Dynamin.
 IPR001401; Dynamin_GTPase.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00350; Dynamin_N 
SMART
 SM00053; DYNc 
PROSITE
 PS00410; DYNAMIN 
PRINTS
 PR00195; DYNAMIN.