CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005530
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Probable acrylyl-CoA reductase AcuI 
Protein Synonyms/Alias
 Acryloyl-coenzyme A reductase AcuI 
Gene Name
 acuI 
Gene Synonyms/Alias
 yhdH; b3253; JW3222 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
188ESTHEYLKSLGASRVacetylation[1]
209AESRPLEKQVWAGAIacetylation[1]
222AIDTVGDKVLAKVLAacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Probably catalyzes the NADPH-dependent reduction of acrylyl-CoA to propanoyl-CoA. 
Sequence Annotation
 NP_BIND 156 159 NADP.
 NP_BIND 178 180 NADP.
 BINDING 41 41 NADP; via amide nitrogen.
 BINDING 198 198 NADP.
 BINDING 242 242 NADP; via amide nitrogen.
 BINDING 256 256 NADP; via carbonyl oxygen.
 BINDING 267 267 NADP.
 BINDING 313 313 NADP.  
Keyword
 3D-structure; Complete proteome; Cytoplasm; NADP; Oxidoreductase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 324 AA 
Protein Sequence
MQALLLEQQD GKTLASVQTL DESRLPEGDV TVDVHWSSLN YKDALAITGK GKIIRNFPMI 60
PGIDFAGTVR TSEDPRFHAG QEVLLTGWGV GENHWGGLAE QARVKGDWLV AMPQGLDARK 120
AMIIGTAGFT AMLCVMALED AGVRPQDGEI VVTGASGGVG STAVALLHKL GYQVVAVSGR 180
ESTHEYLKSL GASRVLPRDE FAESRPLEKQ VWAGAIDTVG DKVLAKVLAQ MNYGGCVAAC 240
GLAGGFTLPT TVMPFILRNV RLQGVDSVMT PPERRAQAWQ RLVADLPESF YTQAAKEISL 300
SEAPNFAEAI INNQIQGRTL VKVN 324 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0043957; F:acryloyl-CoA reductase (NADP+) activity; IEA:EC.
 GO:0008270; F:zinc ion binding; IEA:InterPro. 
Interpro
 IPR013149; ADH_C.
 IPR013154; ADH_GroES-like.
 IPR002085; ADH_SF_Zn-type.
 IPR011032; GroES-like.
 IPR016040; NAD(P)-bd_dom.
 IPR014188; Quinone_OxRdtase_YhdH. 
Pfam
 PF08240; ADH_N
 PF00107; ADH_zinc_N 
SMART
  
PROSITE
  
PRINTS