CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-029670
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 SirtT1 75 kDa fragment 
Protein Synonyms/Alias
 cDNA FLJ90324 fis, clone NT2RP2001817, highly similar to NAD-dependent deacetylase sirtuin-1 (EC 3.5.1.-) 
Gene Name
 SIRT1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
32KFIALSDKEGKLLRNubiquitination[1]
35ALSDKEGKLLRNYTQubiquitination[1]
196RLGGEYAKLCCNPVKubiquitination[1]
210KLSEITEKPPRTQKEubiquitination[1]
298QMENPDLKNVGSSTGubiquitination[2, 3, 4]
307VGSSTGEKNERTSVAubiquitination[2, 3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 444 AA 
Protein Sequence
MCLCSGRKTI LEIYPGQFQP SLCHKFIALS DKEGKLLRNY TQNIDTLEQV AGIQRIIQCH 60
GSFATASCLI CKYKVDCEAV RGDIFNQVVP RCPRCPADEP LAIMKPEIVF FGENLPEQFH 120
RAMKYDKDEV DLLIVIGSSL KVRPVALIPS SIPHEVPQIL INREPLPHLH FDVELLGDCD 180
VIINELCHRL GGEYAKLCCN PVKLSEITEK PPRTQKELAY LSELPPTPLH VSEDSSSPER 240
TSPPDSSVIV TLLDQAAKSN DDLDVSESKG CMEEKPQEVQ TSRNVESIAE QMENPDLKNV 300
GSSTGEKNER TSVAGTVRKC WPNRVAKEQI SRRLDGNQYL FLPPNRYIFH GAEVYSDSED 360
DVLSSSSCGS NSDSGTCQSP SLEEPMEDES EIEEFYNGLE DEPDVPERAG GAGFGTDGDD 420
QEAINEAISV KQEVTDMNYP SNKS 444 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0070403; F:NAD+ binding; IEA:InterPro. 
Interpro
 IPR003000; Sirtuin.
 IPR026590; Ssirtuin_cat_dom. 
Pfam
 PF02146; SIR2 
SMART
  
PROSITE
 PS50305; SIRTUIN 
PRINTS