CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-032150
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Delta(24)-sterol reductase 
Protein Synonyms/Alias
 cDNA FLJ53870, highly similar to 24-dehydrocholesterol reductase (EC1.3.1.-) 
Gene Name
 DHCR24 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
46EWKEQGSKTFMCTGRubiquitination[1, 2]
213GLEAICAKFTHESQRubiquitination[1, 2, 3, 4]
260NSIGNYYKPWFFKHVubiquitination[1, 3, 5]
272KHVENYLKTNREGLEubiquitination[1, 2, 4, 5, 6, 7]
326PPKISLLKLTQGETLubiquitination[1, 2, 3, 4, 5, 7]
335TQGETLRKLYEQHHVubiquitination[1, 3]
418SCMRQLEKFVRSVHGubiquitination[1, 2]
451FDGSLYHKLREKLGCubiquitination[3, 4, 7]
455LYHKLREKLGCQDAFubiquitination[1, 2, 4]
468AFPEVYDKICKAARHubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [7] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 475 AA 
Protein Sequence
MGAGEQNRQS AHCVQGICGY LEGDEEGEEG EVRSTQVREW KEQGSKTFMC TGRPGWLTVS 60
LRVGKYKKTH KNIMINLMDI LEVDTKKQIV RVEPLVTMGQ VTALLTSIGW TLPVLPELDD 120
LTVGGLIMGT GIESSSHKYG LFQHICTAYE LVLADGSFVR CTPSENSDLF YAVPWSCGTL 180
GFLVAAEIRI IPAKKYVKLR FEPVRGLEAI CAKFTHESQR QENHFVEGLL YSLDEAVIMT 240
GVMTDEAEPS KLNSIGNYYK PWFFKHVENY LKTNREGLEY IPLRHYYHRH TRSIFWELQD 300
IIPFGNNPIF RYLFGWMVPP KISLLKLTQG ETLRKLYEQH HVVQDMLVPM KCLQQALHTF 360
QNDIHVYPIW LCPFILPSQP GLVHPKGNEA ELYIDIGAYG EPRVKHFEAR SCMRQLEKFV 420
RSVHGFQMLY ADCYMNREEF WEMFDGSLYH KLREKLGCQD AFPEVYDKIC KAARH 475 
Gene Ontology
 GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
 GO:0008762; F:UDP-N-acetylmuramate dehydrogenase activity; IEA:InterPro. 
Interpro
 IPR016169; CO_DH_flavot_FAD-bd_sub2.
 IPR016166; FAD-bd_2.
 IPR006094; Oxid_FAD_bind_N. 
Pfam
 PF01565; FAD_binding_4 
SMART
  
PROSITE
 PS51387; FAD_PCMH 
PRINTS